NEW BRUNSWICK SCIENTIFIC BioFlo 415, Manual To Operations

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43
New Brunswick Scientific User’s Guide
Figure 17: Calibration Screen (pH)
9. Rinse the pH electrode with distilled water.
10. Immerse pH electrode into a second pH buffer solution which is several pH units
above or below pH 7.00 (e.g., pH 4.00) and allow a few minutes for the system to
equilibrate.
11. Similar to step 7 above, touch the SET SPAN edit box. Use the touchpad that
opens to enter the value of the second buffer solution (e.g., 4.00), then press the
OK button.
12. When the CURRENT VALUE reading stabilizes, press the SET SPAN button.
13. To ensure accuracy, repeat Steps 4-11 a few times, using the same two buffer
solutions.
#
NOTE:
The pH calibration should be checked after sterilization, immediately prior
to inoculation. Take a sample from the vessel and compare the pH value
displayed on the control cabinet screen to the pH recorded by an external
pH meter. Any discrepancy should be adjusted with the
SET ZERO
procedure.
6a. Press
pH here...
6b. ...and pH
appears here.
7. Touch the SET
ZERO edit box. Use
the touchpad that
opens to enter 7.0,
then press the
touchpad’s OK
button.
8. When the
CURRENT VALUE
reading stabilizes,
press the
SET ZERO button.