NEW BRUNSWICK SCIENTIFIC BioFlo 415, Manual To Operations

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37
New Brunswick Scientific User’s Guide
6.2.14 Sterilization Screen
Pressing the Sterilize button at the bottom of any screen allows the operator to
open the STERILIZATION screen (
see Figure 22
). This screen provides access
to an automatic sterilization sequence that controls the devices involved in the
sterilization-in-place of the fermentor.
The sterilization sequence used is based upon the standard sterilization methods
currently used by New Brunswick Scientific. This sequence uses the following
parameters to automatically control the heating and cooling of the vessel:
Table 3: Sterilization Sequence
User-Definable Parameter Definition
YES NO
Drain Time Amount of time valves are open to prime the jacket 9
Heat A Vessel contents are heated via band heater. 9
Heat B Temp Temperature at which steam is passed through filters
to condensate connections
9
Sterilization Temp Temperature at which vessel is held for sterilization 9
Sterilization Time Time vessel is held at sterilization temperature 9
Cool A Time from sterilization to (10
o
C)
9
Cool B Temp Temperature from Cool A to (1
o
C)
9
Growth Temp Temperature at which system transitions back into
growth phase 9
Figure 22: Sterilization Screen
For details on using the STERILIZATION screen,
see Section 8
.
This diagram
represents the
various phases of the
sterilization
sequence. The
current phase is
named and
highlighted in green.
See Figure 33 in
Section 8.4 for
details.