NEW BRUNSWICK SCIENTIFIC BioFlo 415, Manual To Operations

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BioFlo 415
y
M1360-0050 User’s Guide
7
7
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7.1 pH Probe Inspection
Inspect probe for possible shipping damage. If damage is observed, notify the New
Brunswick Scientific Service Department immediately.
If you have a liquid-filled probe, check the level of the reference electrolyte. It should
be about 1cm below the filling orifice. To add reference electrolyte, take the filling
pipette (P0740-4820) and fill it with Viscolyte B (P0860-0130) electrolyte.
Check the electrode tip for trapped air bubbles. To remove any air bubbles, hold the
electrode upright and shake gently.
NEVER REST THE PROBE ON ITS TIP.
#
NOTE:
Both chambers of the electrode are filled with the same reference
electrolyte, for a total volume of approximately 30 mL. For normal
operation, remove the rubber T stoppers if the probe is so equipped,
saving them for use during sterilization.
7.2 pH Probe Calibration
#
NOTE:
Calibrate the pH probe before sterilizing it in the vessel.
1. If you have not already done so, connect the pH probe to the pH connector on the
control cabinet (
see Figure 1a
), using the appropriate cable.
2. Turn the main power switch ON .
3. Press the CALIBRATION button to display the CALIBRATION screen.
#
NOTE:
The pH probe is calibrated using two external buffer solutions of known
pH, usually 7.00 and 4.00.
4. Rinse the pH electrode with distilled water, then immerse it into pH 7.00 buffer
solution and allow a few minutes for the system to equilibrate.
5. Open the CALIBRATION screen (
see Figure 17
, repeated below for easy
reference). Steps 6-8 are indicated as callouts around this screen: