NEW BRUNSWICK SCIENTIFIC BioFlo 415, Manual To Operations

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102
BioFlo 415
y
M1360-0050 User’s Guide
5-normal solutions make a good trade-off between moderate addition volume
and good control characteristics. The correct tubing diameter varies a little
with process, but inside diameters as small as 0.2 mm sometimes eliminate
overcontrol while supplying sufficient liquid during high-demand culture
phases.
#
NOTE:
Whatever the tubing ID, the tubing wall thickness must be 1/16-inch
(1.6 mm).
NBS suggests that you begin with the supplied tubing, which is correct for
most applications. If the system oscillates, reduce the tubing ID where it
passes through the pump. Use commonly available step-up/step-down adapters
and narrower bore tubing to make the tubing modifications, if required.
Consult Table 9, the flow rate/tubing size chart, for further information.
16.3 Inoculation
When you are ready to inoculate the media to start a batch, observe aseptic techniques
to inject the inoculum, using a syringe, through the self-sealing rubber seal of the
12mm or 19mm port septum.
16.4 Launch BioCommand (if present)
Start
BioCommand
on your PC, reset the EFT (Elapsed Fermentation Time) to zero,
and make appropriate program selections to begin logging data. Consult your
BioCommand
user manual for more details.
16.5 Sampling Procedure
The sampler assembly (
see Figure 62 on the following page
) must first be autoclaved:
1.
Apply silicon grease to the
underside only
of the sanitary gasket under the sample
needle cap, to be sure the gasket will stay seated in the sampler needle cap when
you remove it.
2. Wrap the sampler assembly in an autoclavable cloth, foil or paper.
3. Sterilize the assembly in the autoclave for 20 minutes at 121°C.