NEW BRUNSWICK SCIENTIFIC BioFlo 415, Manual To Operations

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52
BioFlo 415
y
M1360-0050 User’s Guide
14. Place the two-position sparge valve into the
sterilization
position: pull the valve
ring out and, as you hold it out, pull the sparger tube up as far as it will go (
see
Figure 30c below
). This allows the steam generated in the vessel to sterilize the
filter. Failure to move this valve into the sterilization position will result in a
failure to sterilize the inlet filter.
15. Make sure silicon grease has been applied to the underside (only) of the
harvest/sample port’s sanitary gasket under the cap.
#
NOTE
:
Be sure to move the sparger tube back into the sparge position, and
to open the exhaust condenser needle valve when sterilization is
over.
The unit is now ready to be sterilized.
Figure 30c: Moving Sparge Valve for Sterilization
8.2.1 Priming the Water Jacket
To fill the water jacket:
1.
After the tubing and water supply have been connected, make sure the RTD
cable is plugged into the controller.
2. Set the temperature loop control mode to OFF .
3. Check that the temperature reading is higher than 5°C.
4. Allow water to enter the piping system; it will stop at solenoid valve TY-
5K.
5. Set the temperature control mode to
AUTO
.
VALVE IN
SPARGE
POSITION
VALVE IN
STERILIZE
POSITION
Ý
AIR IN
AIR OUT
Û
STEAM OUT
Û
Ü
STEAM OUT