NEW BRUNSWICK SCIENTIFIC BioFlo 415, Manual To Operations

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BioFlo 415
y
M1360-0050 User’s Guide
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3.1 System
BioFlo 415 is a versatile sterilizable-in-place (SIP) fermentor that provides a fully
equipped system in one compact package. It can be employed for batch or continuous
culture with process control of pH, dissolved oxygen (DO), agitation, temperature,
pump feed, antifoam, level and addition analog/digital inputs and outputs.
3.2 Vessels
The fermentation vessels are designed for total volumes of 7, 14 and 19.5 liters.
Each stainless steel vessel consists of a headplate, view window and magnetic drive
agitation with Rushton impellers. Ports are provided in the headplate for the following
purposes: inoculation; base and acid addition; a thermowell for a resistance
temperature detector (RTD); a foam probe; a sparger; a harvest tube; a sampling tube;
an exhaust condenser; and dissolved oxygen (DO) and pH electrodes. The drive
bearing housing is also located on the headplate
(see Figures 3a, 8, 9 and 10)
.
3.3 Agitation System
A removable agitation motor located on top of the bearing housing on the headplate is
connected to the agitation shaft with a magnetic drive coupling.
The motor will provide a speed range from 50 to 1000 RPM. The process-control
software ensures agitation speed control from
±
1 RPM to
±
5 RPM throughout the
speed range.
It is possible to cascade Dissolved Oxygen (DO) to Agitation (AGIT) so the agitation
speed will vary between the user-specified minimum and maximum setpoints in order
to maintain the set percentage of DO.
(See Section 11 for further information on
setting up cascades.)
Default P & I (proportional & integral) values are preset at the factory.
We strongly
recommend that you maintain the factory-set parameters.