NEW BRUNSWICK SCIENTIFIC BioFlo 415, Manual To Operations

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51
New Brunswick Scientific User’s Guide
8.2 Sterilization
#
NOTE
:
Recommended sterilization parameters are 45 minutes at 121.5
°
C.
This procedure utilizes the temperature control elements of the system. Be sure to
follow this checklist prior to initiating sterilization:
1.
Make sure the main water line is connected to
CUSTOMER WATER IN
on the side of
the cabinet prior to turning the power on to the unit.
2.
The vessel jacket must be primed with water
(
see Section 8.2.1
).
3. Your water supply pressure must be at least 10 PSIG (0.69 bar or 0.7 kg/cm
2
).
Failure to supply this minimum water pressure will result in failure of the unit to
reach 121.5ºC during sterilization.
4. On the side of the cabinet, make sure the
CUSTOMER WATER OUT
and
CUSTOMER
EXHAUST
are going to the drain.
5. On the side of the cabinet, close the exhaust condenser water needle valve prior to
initiating the sterilization cycle.
6. Behind the vessel, make sure the vessel rupture disk and the vessel jacket release
valve lines are going to the drain.
7. Confirm that the sparge (inlet) and exhaust (outlet) filters are placed in the filter
housings.
#
NOTE
:
Filters must be replaced after 8-10 sterilization cycles.
8. Insert any probes (the calibrated pH probe and/or the DO, Foam and/or Level
probes) into the vessel. Connect the probe cables to the proper jacks on the
cabinet.
9. Verify that mineral oil or glycerin has been added to the thermowell and that the
RTD temperature probe is properly inserted and connected.
10. Be sure to turn agitation ON (we recommend an agitation setpoint of 150-250
RPM). Without agitation during the sterilization cycle, no mixing will occur and
the heater will just turn on and off, creating hot spots in the vessel.
11. Close the drain valve. Check that all ports on the headplate are plugged.
12. Make sure that all tri-clamp connections are finger-tight.
13. Open the 19mm septum/liquid charging port cap and add water and/or non heat-
labile media. Make allowances for inoculum to be added later. Replace the fill
port cap and tighten it securely.
#
NOTE
:
Allow for a loss of up to one liter of media during the sterilization cycle.