Multitron - Operating Manual
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20 August 2020
Page
39
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158
4.2
Direct Steam Humidification
Part of the medium can evaporate, in particular in case of small vol-
umes of work (e.g. when using microtitre and deep well plates) and
long cultivation processes. To reduce evaporation, the device can be
fitted with a direct steam humidification. This regulates the humidity
in the incubation chamber to a defined setpoint and thus ensures a
consistent culture volume.
4.2.1
Setup and Function
The direct steam humidification is mounted on the rear of the casing.
A controlled pump feeds water dropwise into an evaporator chamber
via a feed hose. The water evaporates and is then fed into the incu-
bation chamber.
There are three possibilities to supply the water:
Unpressurised from a water tank
From a pressurised pipe with inlet pressure of max. 0.3 bar
From a pressurised pipe with inlet pressure of max. 6 bar in
combination with the pressure elimination unit (see chapter
4.3 "Pressure Elimination Unit for Direct Steam Humidifica-
tion", page 42)
To prevent condensation inside the device, the device features several
heating elements (door heating, door seal heating and floor heating).
These are activated automatically, as soon as the direct steam humid-
ification is switched on.
For a device without cooling on top, the device is additionally
equipped with a cover (H = 40 mm) to reduce condensation on the
ceiling.
The humidity in the incubation chamber is measured and regulated
using a humidity sensor. This is located in the rear wall of the incuba-
tion chamber.