FEI Quanta FEG 250, User'S Operation Manual, Page: 210 / 228

System Options: Image Analysis
9-62
Image Analysis
The Morphologi software is used for the image processing of saved
images and calculation of morphological data of particles. The
Morphologi SW is installed on the Support PC. Detailed description of
the image processing and particle calculation can be found in the
Morphologi user manual.
In case of issues with Morphologi SW, contact Malvern Instruments
service centre:
phone: +44 1684 891800 (6:30 to 18:00 UK time during weekdays)
email: Helpdesk@malvern.com
MEASUREMENT ACCURACY VERIFICATION
Use the 300 nm and 1 µm polystyrene latex spheres standards to
verify the accuracy of the calculation. Both standards are located in
the ITO slide test sample kit.
Use Mapping tool and save appropriate number of images to have at
least 80 spheres calculated for both the 300 nm and 1 µm standard.
Contrast and brightness of images should be like in the following
examples.
FIGURE 9-52 IMAGES OF 300 nm and 1 µm SPHERES
Load images to Morphologi and set Image Processing to discriminate
spheres from background. Check and tune settings in several
pictures.