FEI Quanta FEG 250, User'S Operation Manual, Page: 143 / 228

Maintenance and Troubleshooting: Refilling the Water Bottle
8-11
Refilling the Water Bottle
The water bottle in the instrument typically needs to be filled about
once a month if the instrument is used on a regular basis at a high
pressure. The water reservoir is located in the rear of the column
console, beneath the frame. To fill the bottle, do the following:
1. Vent the system.
2. Turn off any gas connected to the gas inlet.
3. Run the procedure 154 - Water Bottle Venting (see Chapter 6).
4. Disconnect the quick-coupler and pull out the water bottle.
5. Remove the rubber plug and refill with distilled water (not de-
ionized) until 1/3 full.
6. Mount the rubber plug and install the water bottle in the reverse
order of that described above.
7. Pump the system. Switch to LoVac / ESEM mode to force
automatic purging to flush any air out of the bottle and connecting
tubes.
Note:
The first time the system is pumped to LoVac / ESEM mode after
filling the bottle, Auto-purging may be erratic until the bottle pressure
has steadied. The removal of all the gas from the liquid must be
accomplished before good imaging is possible. This is done correctly
when no bubbles are produced in the water when increasing the
pressure in the chamber.
The heating plate under the bottle should be warm.