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GC Maintenance
2. Position the tool as shown above on a flat, clean surface. Use clean
laboratory tissue on the surface. Tighten the septum support finger-tight.
Holding the tool with a 5/8
″
wrench, give the septum support an extra 1/3 to
1/2 turn past finger-tight. Now unscrew the septum support, which now has
the ferrule and insert seated in it. If there is any graphite extruded past
bottom of the septum support, cut it off with a blade or sharp knife. Carefully
wipe off any graphite flakes, which may adhere to the insert or septum
support. Gripping the septum support unit with a piece of laboratory tissue,
carefully put this unit in the 1079 injector and tighten the septum support 1/6-
turn past finger-tight.
3. Use tweezers to place a new septum over the septum support.
NOTE: If the septum has a Teflon
face, place the Teflon face toward the
column (down).
4. Place the injector nut on the injector and tighten by hand until you feel some
resistance, then tighten an extra 1/4 turn using the injector nut wrench.
NOTE: After the injector nut has been replaced, check the split vent and septum
purge flow rates to ensure these values have not changed.
5. Condition the insert by setting the 1079 Injector to the split mode and purging
with carrier gas for 30 min at 300
°
C.
Clean the Glass Insert
Glass inserts must be clean and free from sample residue and particulate matter
(such as bits of septum rubber or graphite). Follow these steps to clean the glass
insert in the 1079 Injector.
1. Remove the glass insert. It is safest to cool the injector and column ovens to
50 °C before removing the insert.
WARNING:
BURN HAZARD
Use care when removing inserts from the injector. Inserts can be at high
temperatures and are likely hot. Place hot inserts on a clean glass or metal
surface only.
2. To clean glass inserts, use one of the following procedures (the choice of
cleaning procedure depends upon the nature of samples injected):
3. Rinse the inserts with solvent or soak the inserts in hot acid.
4. Heat them in a glass-annealing oven (to 500 °C) or pass the inserts through
the flame of a Bunsen burner.
5. Wash in a 1:1:1 mixture of methanol:methylene chloride:hexane in an
ultrasonic cleaner for 30-60 min, then dry the inserts in an oven.
NOTE: For the 2 mm glass wool packed glass insert, remove the glass wool by
blowing compressed gas in the end of the insert. Clean the insert following one of
the procedures listed above. Repack the insert with deactivated glass wool
(10-20 mg). Leave
∼
1.5 cm of the bottom of the glass insert unpacked. The
capillary column will be inserted in this empty space when re-installed in the
injector body.
