Biotek Synergy LX, User Manual

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Absorbance Testing | 64
Absorbance Testing
Applies only to models with absorbance capability
BioTek developed a series of tests for the absorbance system using a combination of solid
state Absorbance Test Plates and liquid plates. An advantage of running liquid tests is the
liquid in the wells has a meniscus, whereas the test plate’s neutral density glass filters do
not. The test plates and the materials used for creating the liquid plates are available for
purchase from BioTek. See
Optional Accessories on page 4.
To qualify the absorbance system for the Synergy LX , you should perform:
l
Absorbance Liquid Test 1 and Absorbance Plate Test (using PN 7260522) or
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Absorbance Liquid Test 2
Optionally, to qualify operation in the UV range, you should also perform:
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Absorbance Liquid Test 3 or Absorbance Plate Test at 340 nm (using PN
7260551)
BioTek Absorbance Test Plates
Absorbance Test Plate PN 7260522 uses NIST-traceable neutral density filters to confirm
absorbance specifications in the visible range (400–800 nm). This test plate also contains
precision-machined holes to verify mechanical alignment. Absorbance Test Plate PN
7260551 uses NIST-traceable neutral density filters to confirm absorbance specifications in
the UV range (340 nm).
Every test plate comes with a Test Plate Calibration Certificate, containing a table with
Absorbance OD Standards for each filter at each wavelength supported by the plate.
Before the Absorbance Plate Test can be performed, the OD Standard values must be
entered into Gen5. Enter and save these values once initially, and then update them
annually when the test plate is recertified by BioTek.
Check the calibration due date on the test plate's label. If the test plate is overdue for
recalibration, contact BioTek to schedule service.
Test Method
The Absorbance Plate Test is conducted using Gen5 software ( System > Diagnostics > Test
Plates ) to confirm mechanical alignment and optical density accuracy, linearity, and
repeatability. When complete, a results report displays Pass or Fail for each individual test.
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Peak Absorbance: The PN 7260522 test plate contains a glass filter in position C6
that is used to check the wavelength accuracy of the absorbance monochromator.
The filter is scanned across a specified wavelength range in 1-nm increments. The
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.