Absorbance Liquid Tests
|
95
Tube Number
1
2
3
4
5
6
7
8
9
10
Volume of Original
Concentrated Solution
(mL)
20
18
16
14
12
10
8
6
4
2
Volume of 0.05% Tween
Solution (mL)
0
2
4
6
8
10
12
14
16
18
Absorbance expected if
original solution is 2.0 at
200 μL
2.0
1.8
1.6
1.4
1.2
1.0
0.8
0.6
0.4
0.2
The choice of dilutions and the absorbance of the original solution can be varied. Use
this table as a model for calculating the expected absorbances of a series of dilutions,
given a different absorbance of the original solution.
2. Pipette 200 µL of the concentrated solution from Tube 1 into each well of the first
column, A1 to H1, of a new flat-bottom microplate.
3. Pipette 200 µL from each of the remaining tubes into the wells of the corresponding
column of the microplate (Tube 2 into wells A2 to H2, Tube 3 into wells A3 to H3, and so
on).
After pipetting the diluted test solution into the microplate and before reading
the plate, we strongly recommend shaking the plate for four minutes. This will
allow any air bubbles in the solution to settle and the meniscus to stabilize.
Alternatively, wait 20 minutes after pipetting the test solution before reading the
plate.
4. Create a Gen5 experiment based on the
Synergy LX Abs Test 2
protocol and read the
plate. When prompted, rotate the plate 180 degrees.
5. When finished:
l
Save the experiment. Refer to the instructions on page 69 to perform calculations
and determine pass/fail.
l
Troubleshooting tips are provided on page 72.
l
Test descriptions are provided on page 67.
Synergy LX User Manual
1501000N Revision A
BioTek Instruments, Inc.
Summary of Contents for Synergy LX
Page 46: ...22 Chapter 2 Installation Synergy LX User Manual 1501000N Revision A BioTek Instruments Inc ...
Page 55: ...Filter Cubes 31 Synergy LX User Manual 1501000N Revision A BioTek Instruments Inc ...
Page 139: ...Sample System Report 115 Synergy LX User Manual 1501000N Revision A BioTek Instruments Inc ...
