Biotek Synergy LX, User Manual

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Luminescence Test | 82
2. On the Harta plate’s Calibration Certificate, locate the NIST measurement for the A2
position and convert it to attomoles:
(A2 NIST measurement*0.02884)
3. Calculate the signal-to-noise ratio:
(A2–Mean of the buffer cells)/(3 * Standard deviation of
buffer cells)
4. Calculate the detection limit:
A2 NIST measurement in attomoles/signal-to-noise ratio
Pass/Fail Criteria
l
If the reader is equipped with the low-noise PMT, the detection limit must be ≤ 75
amol to pass.
l
If the reader is equipped with the red-shifted PMT, the detection limit must be ≤
500 amol to pass.
If you do not know which PMT is installed, (#49984 = low-noise PMT; #49721 = red-
shifted PMT, please contact Technical Support.
Gen5 Protocol Reading Parameters
The information in this section represents the recommended reading parameters for the
referenced Gen5 protocol(s).
Synergy LX _LumTest_Harta.prt
Parameter Default Setting
Plate Type: 8030015 Harta with 8032028 adapter
Delay Step: 3 minutes
Read Step 1:
Detection Method:
Luminescence
Read Type:
Endpoint
Step Label: Reference well A2
Read Wells: A2
Filter Set: LUM
Gain: 150
Integration Time: 0:10.00 MM:SS.ss
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.