Biotek Synergy LX, User Manual

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96 | Chapter 6 : Instrument Qualification Procedures
Absorbance Liquid Test 3 (optional)
Absorbance Liquid Test 3 is provided for sites requiring proof of linearity at 340 nm.
This test is optional because the Synergy LX has good "front end" linearity throughout
its wavelength range. As an alternative, the 340 nm Absorbance Test Plate (PN
7260551) may be used for this test.
Materials
Manufacturer part numbers are subject to change.
l
New 96-well, clear, flat-bottom microplate (Corning Costar #3590 recommended)
l
Calibrated hand pipette(s)
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Beakers and graduated cylinder
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Precision balance with readability to 0.01 g
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Buffer solution described below
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The Gen5 protocol Synergy LX Abs Test 3.prt , described on page 69
Buffer Solution
l
Deionized water
l
Phosphate-Buffered Saline (PBS), pH 7.2–7.6, Sigma tablets, #P4417 (or
equivalent)
l
β-NADH Powder (β-Nicotinamide Adenine Dinucleotide, Reduced Form) Sigma
bulk catalog number N 8129, or preweighed 10-mg vials, Sigma number N6785-
10VL (or BioTek PN 98233). Store the powder according to the guidelines on its
packaging.
1. Prepare a PBS solution from the Sigma tablets.
2. In a beaker, mix 50 mL of the PBS solution with 10 mg of the β-NADH powder and mix
thoroughly. This is the 100% Test Solution .
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.