Biotek Synergy LX, User Manual

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Absorbance Liquid Tests | 97
Test Procedure
1. Prepare the 75% Test Solution by mixing 15 mL of the 100% Test Solution with 5 mL of
the PBS Solution.
2. Prepare the 50% Test Solution by mixing 10 mL of the 100% Test Solution with 10 mL of
the PBS Solution.
3. Carefully pipette the three solutions into a new 96-well microplate:
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150 µL of the 100% Test Solution into all wells of columns 1 and 2
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150 µL of the 75% Test Solution into all wells of columns 3 and 4
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150 µL of the 50% Test Solution into all wells of column 5 and 6
After pipetting the diluted test solution into the microplate and before reading
the plate, we strongly recommend shaking the plate for four minutes. This will
allow any air bubbles in the solution to settle and the meniscus to stabilize.
Alternatively, wait 20 minutes after pipetting the test solution before reading the
plate.
4. Create a Gen5 experiment based on the Synergy LX Abs Test 3 protocol and read the
plate.
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Save the experiment. Refer to the instructions on page 69 to perform calculations
and determine pass/fail.
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Troubleshooting tips are provided on page 72.
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Test descriptions are provided on page 67.
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.