Biotek Synergy LX, User Manual

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Operate the Reader Using the Touchscreen | 35
The BCA Protein and Bradford protocols shown above come predefined on absorbance-
capable models.
Up to 60 uniquely named protocols can be saved on the reader at one time, excluding the
predefined Take3 protocols.
Protocols created through the touchscreen are limited to a single endpoint read step at
one or two wavelengths and up to 12 blank wells. Use Gen5 if your assays require more
complex reading methods or plate layouts.
The tabbed sections in the middle of the Main Menu contain the protocols created for each
of the available detection methods and the Take3 plate (if used). To run an existing
protocol, simply tap its name, define the columns to be read if not reading the full plate,
and then tap
Start .
The right side of the Main Menu provides access to the following features:
l
Quick: Define and run an Absorbance, Fluorescence, or Luminescence read, or
perform a standalone shake. Quick reads do not support shake or delay actions,
and they read the full plate. See page 37.
l
Protocol: Edit, create (and save), delete, and copy protocols. Through this menu
option, protocols support shake and delay actions and, at run time, allow you to
select a subset of the full number of plate columns to read.
l
Results: Provides access to results for the 12 most recently run protocols. Tap a
protocol name to view its results, and then tap the screen to cycle through the
available data sets (e.g., raw data, delta OD, blanked data).
l
Instrument: Run a system test, define date/time settings, enable output to a
printer and/or USB flash drive, define filter cubes (models with
fluorescence/luminescence capability), and configure a Take3 plate (models with
absorbance capability).
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.