Biotek Synergy LX, User Manual

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50 | Chapter 3 : Getting Started
1. In the Gen5 Task Manager, select Experiment > Create using an existing protocol .
2. Select the desired protocol and click OK .
3. Select a plate in the menu tree and click the Read Now button.
4. When the read is complete, measurement values appear in Gen5. Select the desired
data set from the Data list.
5. Select File > Save and give the file an identifying name.
Features Supported by Synergy LX That Require Gen5
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Reader qualification
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Performing fluorescence reads in more than one color
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Kinetic reads
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Area and spectrum scan
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Nonstandard plate types
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Partial plate reading beyond column-based
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Automatic export to Microsoft Excel
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Extensive data reduction options, including cutoff and transformations
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Ability to define standard wells and generates curves
Recommendations for Optimum Performance
General
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Microplates should be clean and free from dust or bottom scratches. Use new
microplates from sealed packages. Do not allow dust to settle on the surface of
the solution; use microplate covers or seals when not reading the plate. Filter
solutions to remove particulates that could cause erroneous readings.
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Although the Synergy LX supports standard flat, U-bottom, and V-bottom
microplates, when using absorbance, the reader achieves optimum performance
with flat-bottomed wells. See
Appendix A, Specifications for more information on
the supported plates.
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.