Biotek Synergy LX, User Manual

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48 | Chapter 3 : Getting Started
Plate Shaking Options
The Synergy LX supports linear, orbital, and double-orbital shaking plate shaking, with user-
selected amplitude from 1 mm to 6 mm, in 1 mm steps. Frequency ranges from about 18
Hz to about 6 Hz, depending on the selected amplitude.
l
Define a stand-alone shake from the Main Menu by tapping Quick and then Shake .
l
Add a shake in a protocol by tapping the Activity Before Read field and selecting
Shake .
Getting Started with Gen5 Software
BioTek Gen5 software supports all Synergy LX reader models. This section provides brief
instructions creating protocols and experiments. For more information, refer to
publications provided with Gen5 and the Gen5 help system (Help > Help Topics).
Protocols and Experiments
In Gen5, a protocol contains instructions for controlling the reader and (optionally)
instructions for analyzing the data retrieved from the reader. At a minimum, a protocol
must specify the procedure for the assay you wish to run. After creating a protocol, create
an experiment that references the protocol. Run the experiment to read plates and analyze
the data.
To create a protocol:
1. Optional, but recommended: Create the protocol with the reader connected to the
computer and turned on.
2. In the Gen5 Task Manager, select Protocol > Create New .
3. Open the Procedure dialog. If prompted to select a reader, select Synergy LX and click
OK.
4. Select a Plate Type to match your assay plate.
Gen5 stores measurements and other characteristics for individual plate types in
a database. It is essential that you select the plate type to match the assay plate.
Otherwise, results may be invalid.
5.
Add a Read step to the Procedure.
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.