Biotek Synergy LX, User Manual

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Fluorescence Testing | 80
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If the Corners Test continues to fail, the hardware may be misaligned. Contact
Technical Support .
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Review the pipetting instructions to verify the plate was correctly prepared.
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Does the Plate Type setting in the Gen5 protocol match the plate you used?
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The Read steps in the protocols use the Gen5 Automatic Gain Adjustment feature to
determine optimum sensitivity values for the plate. If an Auto Gain Result value is
outside the range of 30–200, this may indicate a problem.
If the value is less than 30:
o
The stock solution/dilution concentrations may be too high. Try creating fresh
solutions/dilutions, and rerun the test using a new, clean plate.
o
If all of the tests are passing but the Gain value is low, a PMT in your reader
may just be very sensitive. Contact
Technical Support to confirm that this may
be the case.
If the value is greater than 200:
o
The stock solution/dilution concentrations may be too low. Try creating fresh
solutions/dilutions, and rerun the test using a new, clean plate.
o
The PMTs or optical path(s) may be deteriorating, or the optics or other
hardware may be misaligned. Contact
Technical Support .
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.