Biotek Synergy LX, User Manual

Page: 127 / 198

Fluorescence Liquid Tests | 103
BioTek offers a liquid test kit (PN 7160012) containing the microplates and solutions
used in the MUB fluorescence liquid tests.
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Methylumbelliferone (“MUB”) (10-mg vial, BioTek PN 98156)
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Carbonate-Bicarbonate buffer (“CBB”) capsules (BioTek PN 98158)
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100% methanol (BioTek PN 98161)
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A new, clean 96-well solid black plate microplate, such as Corning Costar #3915 or
equivalent.
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Blue filter cube: EX: 360/40, EM: 460/40, Mirror: 400 nm; installed in the reader and
configured in Gen5
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Deionized or distilled water
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Various beakers, graduated cylinders, and pipettes
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Aluminum foil
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(Optional, but recommended) 0.45-micron filter
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(Optional) Black polyethylene bag(s) to temporarily store plate(s)
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Gen5 protocol described on page 74:
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Synergy LX FI_T_MUB.prt
Test Solutions
Filter solutions to remove particulates that could cause erroneous readings. Do not
allow dust to settle on the surface of the solution; use microplate covers or seals when
not reading the plate.
Wrap the vial containing the MUB stock solution in foil to prevent exposure to light.
Discard any open, unused solutions after seven days.
1. Prepare the buffer (CBB) solution:
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(Optional, but recommended) Using a 0.45-micron filter, filter 200 mL of deionized
or distilled water.
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Open and dissolve the contents of two CBB capsules (do not dissolve the outer
gelatin capsule) into 200 mL of the water.
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.