Biotek Synergy LX, User Manual

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Fluorescence Testing | 74
Fluorescence Liquid Tests
Test Methods
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The Corners Test uses fluorescence compounds to verify that the plate carrier is
properly aligned in relation to the fluorescence optics.
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The Sensitivity Test uses a fluorescence compound and buffer solution to test the
fluorescence reading capability of the instrument. The ability to detect specific
compounds at the required limit of detection ensures that the filters, optical path,
and PMT are all in working order. This test verifies that the difference between the
concentration well under investigation and the mean of the median buffer well is
statistically distinguishable.
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The Linearity Test verifies that the system is linear; that is, the signal changes
proportionally with changes in concentration (R
2
value). Proving that the system is
linear allows the Sensitivity Test to be run on two points instead of using serial
dilutions.
The tests presented in this section require specific microplates, solutions,
wavelengths, mirrors, and filters. Your laboratory may require a deviation from some
of these tests. For example, you may wish to use a different fluorescing solution or
microplate.
If deviation from the tests as presented in this section is required, the following
steps should be taken the first time each test is run (e.g., during the Initial OQ):
1. Perform the tests exactly as described on the following pages.
2. Rerun the tests using your particular solutions, filter cubes, microplates, and so
on. If results are comparable, then the results from these tests will be your baseline
for future tests.
3. Document your new test procedure(s), and save all test results.
Gen5 Protocol Reading Parameters
The information in the following tables represents the recommended reading parameters.
It is possible that your tests will require modifications to some of these parameters, such as
the Plate Type (see
Troubleshooting Tips on page 79).
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.