Biotek Synergy LX, User Manual

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79 | Chapter 5 : Instrument Qualification Process
Linearity Test
1. Calculate the Mean of the four wells for each concentration in columns 1–5.
2. Perform linear regression using these values as inputs:
Sodium Fluorescein
x y
1000 Mean of the 1000 pM wells
500 Mean of the 500 pM wells
250 Mean of the 250 pM wells
125 Mean of the 125 pM wells
62.5 Mean of the 62.5 pM wells
Methylumbelliferone
x y
100 Mean of the 100 nM wells
50 Mean of the 50 nM wells
25 Mean of the 25 nM wells
12.5 Mean of the 12.5 nM wells
6.25 Mean of the 6.25 nM wells
3. Calculate the R
2
value; it must be >= 0.9500 to pass.
Troubleshooting
If any tests fail, please try the following suggestions. If the test(s) continue to fail, print the
results and contact
Technical Support .
l
Are the solutions fresh? Discard the plate and any opened, unused test solutions after
seven days.
l
Are the excitation/emission filters clean?
l
Are you using the proper filter cube?
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.