Biotek Synergy LX, User Manual

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Fluorescence Liquid Tests | 101
Mix This SF Solution: With Buffer: To Make:
0.53 mL of 1.3288 mM
stock solution
13.47 mL 50.2 μM
110 µL of 50.2 μM SF 13.89 mL 400 nM
3.5 mL of 400 nM SF 10.5 mL 100 nM
0.46 mL of 100 nM SF 13.54 mL 3.3 nM Corners Test
4.24 mL of 3.3 nM SF 9.76 mL 1 nM
Sensitivity/Linearity
Tests
Test Procedure
1. If you have not already done so, prepare the solutions for the tests you plan to perform.
See Test Solutions on page 100.
2. Pipette the solutions for the Corners and Sensitivity/Linearity Tests into a clean, new 96-
well solid black plate. Refer to the Pipette Map on page 101.
3. Create a Gen5 experiment based on the Synergy LX FI_T_SF.prt protocol described on
page 74.
4. Read the plate, and then save the experiment.
Pipette Map
Seal the plates with foil or store them in black polyethylene bags until use. When using
a clear-bottom plate, if the base of the plate is touched, clean the entire base with
alcohol (95% ethanol) and then wipe with a lint-free cloth. Before placing the plate in
the instrument, blow the bottom of the plate with an aerosol duster.
Corners, Sensitivity, and Linearity (FI) Tests
Using a single-channel pipette:
l
Pipette 200 μL of the 3.3 nM SF solution into the "corner" wells.
l
Pipette 200 μL of buffer into the wells surrounding the SF. (Omit if using a solid
black plate or Greiner SensoPlate).
l
Pipette 200 μL of the 1 nM SF solution into well D7.
l
Pipette 200 μL of the buffer solution into wells C9, D9, and E9.
Using a multichannel pipette with just four tips installed:
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.