Biotek Synergy LX, User Manual

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Fluorescence Liquid Tests | 99
Required Materials
Kits containing the microplates and solutions required by the Liquid Tests are available
for purchase; see Materials for Conducting Liquid Tests on page 5.
Manufacturer part numbers are subject to change.
All Tests:
l
Deionized or distilled water
l
Various beakers, graduated cylinders, and pipettes
l
Aluminum foil
l
(Optional, but recommended) 0.45-micron filter
l
(Optional) Black polyethylene bag(s) to temporarily store plate(s)
l
Gen5 protocols (described starting on page 74):
o
Synergy LX FI_T_SF.prt
o
Synergy LX FI_T_MUB.prt
Corners/Sensitivity/Linearity (FI) Tests
Manufacturer part numbers are subject to change.
The materials listed here are for use with Sodium Fluorescein. Methylumbelliferone
can be used as an alternate or supplemental method for performing these tests. See
page 102.
If using test kit PN 7160013 (see page 5), the buffer and SF are pre-diluted.
l
Buffer:
o
NIST-traceable Sodium Borate Reference Standard (pH 9.18) (e.g., Fisher-
Scientific 1 L Sodium Borate Mfr. #159532, or equivalent),
or
o
Phosphate-Buffered Saline (PBS), pH 7.2–7.6 (e.g., Sigma tablets, Mfr. #P4417,
or equivalent) and pH meter or pH indicator strips with pH range 4 to 10
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.