Zeiss Axioscope 5, Инструкция по эксплуатации

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ZEISS 5 Operation | 5.5 Setting Up for Reflected Light Techniques
3. Slide the C-DIC slider
1
into the 6x20 mm
compensator slot.
3
1
2
4. Place the sample on the stage.
5. Focus the sample.
6. Turn the setting wheel
2
on the C-DIC slider so the structure of interest is visible with
maximum contrast.
à
No further stage rotation is necessary.
7. Optimized the contrast by adjusting the setting screw
3
.
5.5.5 Setting Up for Reflected Light TIC Microscopy
For more information about the method, see chapter Reflected Light TIC Microscopy [
}
60] .
Prerequisite ü The HAL 100 light source is installed [
}
77] .
ü
The microscope is operational.
ü A suitable objective for DIC is installed [
}
67] , e.g. EC Epiplan-Neofluar, Epiplan with the addi-
tional label "DIC" or "Pol".
ü
TIC slider 6x20 mm with the appropriate C-DIC reflector module is available.
ü The illumination is adjusted for reflected light brightfield microscopy [
}
105] .
Procedure 1. Place the sample on the stage.
2. Focus the sample.
3. Swivel the C-DIC module on the reflector turret
into the beam path.
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001 111