Zeiss Axioscope 5, Инструкция по эксплуатации

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5 Operation | 5.4 Setting Up for Transmitted Light Techniques ZEISS
5.4.8.2 Determination of the Polarization Direction
For more information about the method, see chapter Determination of the Polarization Direction
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Prerequisite
ü An eyepiece with cross hair reticle is installed [
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66] .
ü The Pol adjustment sample for polarization microscopy is available.
Procedure 1. Swing the polarizer into the beam path.
2. When using a rotatable polarizer, position it to 0°.
3. Put the analyzer into the slit for the compensator or swing analyzer module on the reflector
turret/slider.
à
The field of view appears dark.
4. Place the Pol adjustment sample on the microscope.
5. Turn the rotary stage until the adjustment sample appears dark.
6. Remove the analyzer from the beam path.
7. Align the reticle of the eyepiece along the split cracks of the adjusting sample.
8. Return the analyzer into the beam path.
9. Remove the adjustment sample.
à The forward direction of the polarizer and analyzer is parallel to the cross hair in the reti-
cle (polarizer east-west, analyzer north-south).
10. Turn the rotary stage with the sample, e.g. a synthetic fiber, until the sample reaches maxi-
mal darkness.
à
The fiber is parallel to one of the two lines in the cross hair orientation.
11. Turn the rotary stage by approx. 45° until the longitudinal axis of the fiber is pointing in
northeast-southwest direction.
à The sample shows the strongest brightness (diagonal position). It can have any color in
this position.
12. Slide in the lambda compensator (possible only if used with screw-in analyzer in tube or in-
termediate plate).
à
The sample changes its color depending on its orientation (northeast-southwest or north-
west-southeast).
5.4.8.3 Measuring Path Differences
For more information about the method, see Measuring Path Differences [
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58] .
Prerequisite ü The correct inter-pupillary distance in the binocular tube is set.
Procedure 1. Accurately position the sample to be examined on the center of the eyepiece reticle.
2. Limit the aperture to a value of about 0.2.
3. Turn the rotary stage until the sample is almost extinguished, i.e.
completely dark , and set
the 45° locking position.
4. Rotate the stage once (by 45°) so that the sample is in a diagonal position (sample be-
comes bright).
5. Determine [
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58] the suitable compensator.
6. Insert the determined compensator into the slot as far as it will go.
7. Use the enclosed operating instructions for measurement preparation and measurement
procedure.
100 Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001