ZEISS
5 Operation | 5.5 Setting Up for Reflected Light Techniques
5
4
7
8
9
11
10
1
2
2
3
6
1
Aperture diaphragm
2
Centering screw (2x)
3
Field diaphragm
4
Reflector turret
5
Knurled ring of the nosepiece
6
Condenser turret
7
Colibri 3 light source
8
Filter slider for reflected light with
blocking position
9
Intensity/LM button
10
RL button
11
TL button
Prerequisite
ü
If used, the mercury vapor short arc lamp of the HBO 100 light source is
.
ü
In the reflector turret, FL P&C reflector modules equipped with respective filter sets are in-
stalled.
ü
Fluorescence protection shield is available.
ü
A suitable objective for fluorescence microscopy is installed.
e.g. EC Plan-Neofluar or Fluar (UV excitation)
ü
The microscope is operational.
ü
The microscope is adjusted for
reflected light brightfield microscopy [
.
Procedure
1. If necessary, remove the compensator from the 6x20 mm slot above the nosepiece.
2. Slide the fluorescence protection shield into the 6x20 mm slot.
3. Swivel in the EC Plan-Neofluar objective on the nosepiece
5
.
4. Initially set transmitted light illumination by pushing the
TL button
11
.
5. If necessary, turn the condenser turret
6
to the
H
(B) position for transmitted light
brightfield (or phase contrast if using a Ph objective).
6. Seek the sample detail to be examined.
7. Keep the light path in the reflected light light source blocked via the blocking position of
the filter slider for reflected light.
8. Switch on the Colibri 3 light source
7
by pushing the
RL button
10
.
9. Press the
Intensity/LM button
9
briefly for less than 1.5 seconds repeatedly to activate
the required LED module or all LED modules of Colibri 3 together.
à
The indicator light of the respective LED module on the Colibri 3 lights up when this
module is switched on.
10. If using the HBO 100 light source, switch on the external power supply and let it warm up
to operational temperature for about 15 minutes.
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001
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