5 Operation | 5.4 Setting Up for Transmitted Light Techniques
ZEISS
10. Turn the knurled knob for vertical adjustment
to lower the condenser until the edge of the lu-
minous-field diaphragm appears in focus
B
.
B
11. Center the luminous-field diaphragm using the
two centering screws on the condenser carrier
C
.
C
12. Open the luminous-field diaphragm until the
edge of the diaphragm just disappears from the
field of view
D
.
D
13. Remove an eyepiece from the binocular tube to adjust the aperture diaphragm (contrast).
14. Look into the tube with the naked eye.
15. Set the aperture diaphragm with the adjusting
lever to between 2/3 - 4/5 of the diameter of
the exit pupil of the objective
E
.
E
à
In most applications, this aperture diaphragm setting provides optimal contrast at almost
ideal resolution, and is therefore the best compromise for the human eye.
16. Reinsert the eyepiece into the binocular tube.
17. Remove the high-contrast sample.
90
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001
