Zeiss Axioscope 5, Инструкция по эксплуатации

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ZEISS 5 Operation | 5.4 Setting Up for Transmitted Light Techniques
5.4.8.5.2 Conoscopy with Bertrand System Module
Prerequisite
ü Strain-free objectives are installed in the nosepiece [
}
67] .
N-Achroplan 50x/0.9 Pol objective or EC Plan-Neofluar 40x/0.9 Pol objective
ü The Pol rotary stage is installed [
}
159] .
ü Pol binocular photo tube is installed or eyepiece with crossline micrometer 14:140 and adjust-
ment aid for polarization microscopy are available.
ü The achromatic-aplanatic 0.9 H Pol condenser or 0.9 Pol condenser is installed.
ü The Bertrand system module is inserted in reflector turret.
ü The D polarizer (rotatable or fixed) is installed.
ü Analyzer slider or D Pol analyzer module in the reflector turret or reflector slider are available.
ü The microscope is adjusted for transmitted light brightfield microscopy [
}
87] .
Procedure 1. Place the sample on the stage.
2. Focus the sample.
3. Swivel the polarizer.
4. If you do not use the Pol binocular photo tube, carry out the following two steps:
− Align the crossline micrometer 14:140 or the eyepiece reticle to the vibration direction of
the polarizer using the
adjustment aid [
}
100] .
− Remove the Pol adjustment aid.
5. Move a selected crystal to the center of the reticle.
6. Swivel in the front lens on the condenser, if necessary.
7. Swivel the 40x or 50x objective into the light path.
8. Focus the sample.
9. Close the luminous-field diaphragm as much as is necessary to prevent the axial figure from
being superimposed by the axial figures of adjacent crystals.
à The smallest crystal extension that can be masked out is 4 μm.
10. Swivel in the Pol Bertrand system module on the reflector turret.
à
The axial figure appears in the field of view.
Info
In the case of uniaxial crystals, the most favorable orientation for conoscopic viewing is ob-
tained with those sample features (e.g. of a thin section) that in orthoscopic viewing change
the brightness as little as possible upon rotating the stage. In this case, the direction of view-
ing and the optical axis are nearly parallel. The same refers also to biaxial crystals, if they are
viewed along or approximately in the direction of one of the two optical axes.
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001 103