Zeiss Axioscope 5, Инструкция по эксплуатации

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5 Operation | 5.4 Setting Up for Transmitted Light Techniques ZEISS
5.4.5 Setting Up for Transmitted Light Phase Contrast Microscopy
Prerequisite
ü The microscope is operational.
ü Phase contrast objectives with the phase rings PhC 1 , PhC 2 or PhC 3 are installed [
}
67] .
ü Condenser with modulator disk with centerable ring diaphragms PhC 1 , PhC 2 and PhC 3 is
installed [
}
168] .
Procedure 1. Swivel the phase contrast objective into the beam path (e.g. Ph1 ).
2. Swivel in the annular phase diaphragm on the condenser’s revolver disk with the same la-
beling as the objective (e.g. Ph1 )
3. Replace one eyepiece [
}
66] with an auxiliary microscope.
4. With the adjusting fixture on the auxiliary microscope, focus the annular phase diaphragm
and the phase ring in the objective exit pupil.
5. Check the centering and the overlap of the
lighter annular phase diaphragm (in the con-
denser) with the darker phase ring (in the ob-
jective).
Both rings must be centered and overlapping
B
.
A B
6. If the overlap is not exact
A
, recenter the lighter annular phase diaphragm.
7. Remove the auxiliary microscope and replace the eyepiece.
Info
To increase the image contrast, a green 32 x 4 interference broadband filter can be mounted
on the field diaphragm or inserted into the color glass carrier (if available).
96 Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001