Zeiss Axioscope 5, Инструкция по эксплуатации

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5 Operation | 5.5 Setting Up for Reflected Light Techniques ZEISS
5.5.2 Setting Up for Reflected Light Darkfield Microscopy Using the KÖHLER Method
For more information about the method, see chapter Reflected Light Brightfield Microscopy Using
the KÖHLER Method [
}
59] .
Prerequisite
ü A reflected light light source is installed [
}
77] .
ü In the reflector turret, an ACR P&C reflector module for reflected light is installed.
ü A suitable objective for RL darkfield microscopy is installed [
}
67] , e.g. Epiplan-Neofluar HD,
EC Epiplan-Neofluar HD, Epiplan HD.
ü The microscope is operational.
ü The illumination is adjusted for reflected light brightfield microscopy [
}
105] .
The field diaphragm image should lie just barely outside of the edge of the field of view to
avoid reflections
Procedure 1. Swing the darkfield ACR P&C reflector module
for reflected light on the reflector turret into
the beam path.
2. Remove the 6x20 mm compensator slider if inserted.
3. Swivel the objective position with the darkfield
HD objective into the beam path.
4. Completely open the aperture diaphragm A .
5. Switch off or remove neutral filters if applicable.
6. Place the sample on the stage.
108 Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001