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3 Product and Functional Description | 3.4 Microscopy and Contrast Methods

ZEISS

3.4.10 Reflected Light TIC Microscopy

The reflected light TIC method (Micro-interferometry; TIC = Total Interference Contrast in the cir-
cular polarized light) is used in imaging and measuring sample structures that exist in different az-
imuths.

Evaluation of the measured values

Fig. 36: Interference stripes

The values

a

(distance between interference stripes) and

b

(offset of the interference stripes along

the step) are determined with the aid of an eyepiece reticle micrometer or with a micrometer eye-
piece.

If working with white light (without an interference filter), set λ = 550 nm. When interference fil-
ters are used, it is important to apply the focal point of their wave lengths.

The measured path difference depends on the aperture and increases with the illumination aper-
ture.

The step height SH is determined with the following formula:

Where

SH = step height in nm

n = refractive index of the environment, mostly air (n = 1)

Δ = phase difference

a = distance between interference stripes

b = offset of the interference stripes along the step

λ = wave length of the illumination in nm

The following correction values must be considered depending on the objective used:

Objective

Correction factor k

5x/0.15

1.0057

10x/0.25

1.0161

10x/0.30

1.0236

20x/0.4

1.0436

20x/0.50 and 50x/0.75

1.0718

50x/0.60

1.1111

50x/0.75 and 100x/0.75

1.2038

50x/0.80

1.2500

60

Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001

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Содержание Axioscope 5

Страница 1: ...Instruction Manual ZEISS Axioscope 5 Axioscope 5 7 MAT Upright Microscope for Routine and Entry level Research...

Страница 2: ...by any in formation or filing system The right to make backup copies for archiving purposes shall remain unaffected thereby Any viola tions may be prosecuted as copyright infringements The use of gene...

Страница 3: ...ated by Radiation 18 2 3 7 Thermal Hazards 18 2 4 Labels and Lights 19 2 4 1 Labels on the Axioscope 19 2 5 Safety Devices and Interlocks 22 3 Product and Functional Description 23 3 1 Main Components...

Страница 4: ...Mechanical Stage 70 4 7 3 Assembling the Motorized Mechanical Stage on the Axioscope 7 Mo torized Material Stand 70 4 8 Assembling the Stage Carrier 71 4 9 Assembling the Condenser Carrier 72 4 10 Ass...

Страница 5: ...etting Up for Reflected Light C DIC Microscopy 110 5 5 5 Setting Up for Reflected Light TIC Microscopy 111 5 5 6 Setting Up for Reflected Light Polarization Microscopy Proof of Bire flectance and Refl...

Страница 6: ...sting the Friction of the Coaxial Knurled Knobs on the Ergonomic Stage Drive 163 10 8 Loading the Reflector Module 164 10 8 1 Assembling Reflector Modules 164 10 8 2 Changing the Filters of a Reflecto...

Страница 7: ...PhC DIC 191 10 14 1 Assembling the Condenser Chromatic aplanatic 0 9 BF DF PhC DIC 191 10 14 2 Centering the Darkfield Diaphragm of the Condenser 193 10 14 3 Centering the Annular Phase Diaphragm of t...

Страница 8: ...se of the microscope This document is an essential part of the microscope and if the microscope is resold the docu ment must remain with the microscope or be handed over to the new owner Axioscope 5 m...

Страница 9: ...ane ously Select Tools Goto Control Panel Air lock Follow a path in the software Enter example pdf in this field Text to be entered by the user Programming and Macros Anything typed in literally durin...

Страница 10: ...dicating dangerous situations and hazards are used in this doc ument DANGER Type and source of danger DANGER indicates an imminently hazardous situation which if not avoided will result in death or se...

Страница 11: ...ountry of manufacture CC is the country code e g DE for Germany CN for China Date of manufacture optionally given adjacent to this symbol Importer Authorized representative in the European Community I...

Страница 12: ...ict with the installa tion requirements of the microscope Safety Data Sheets Observe the enclosed safety data sheets The instructions and guidelines given in the respective safety data sheets must be...

Страница 13: ...service support training and education html contact ZEISS Portal The ZEISS Portal https portal zeiss com offers various services that simplify the daily work with your ZEISS systems machines and soft...

Страница 14: ...by incorrect operation negligence or unauthorized intervention in particular by removing modifying or replacing parts of the microscope or its com ponents cannot be held liable by the device manufactu...

Страница 15: ...lowing table lists the risk grouping of the available light sources illumination units accord ing to the mentioned standard Light source Illumination unit Risk group Colibri 3 3 high risk HBO 100 2 mo...

Страница 16: ...tion must be observed at all times and during all work on and with the microscope Training Authorized ZEISS personnel will provide basic training in operating the microscope As well as in formation on...

Страница 17: ...and Moisture Dirt dust and moisture can impair the microscope s functionality Shut down the microscope whenever it is not used and cover it with a dust protection cover Always cover unused openings p...

Страница 18: ...Decontaminated parts can be sent to ZEISS accompanied by a signed Customer Declaration of Decontamination Wear gloves 2 3 6 Hazards Generated by Radiation Optical Radiation Hazards Gas discharge ligh...

Страница 19: ...ely replace damaged or illegible warning labels In case a label is missing please contact your ZEISS service representative for free of charge re placement 2 4 1 Labels on the Axioscope 1 2 5 3 4 6 Fi...

Страница 20: ...sition of the warning label on the Axioscope 7 stand using the motorized 80x60 me chanical stage 6 Fig 5 Position of the warning label on the power supply for two HAL 100 lamps 6 9 Fig 6 Position of t...

Страница 21: ...mber label 3 Microscope type label valid for the Axioscope 5 microscopes Microscope type label valid for the Axioscope 5 7 MAT microscopes 4 EU Representative Carl Zeiss Microscopy GmbH Carl Zeiss Pro...

Страница 22: ...IEC 62471 Risk group 3 according to IEC 62471 WARNING Possibly hazardous optical radia tion emitted from this product Do not look at operating lamp Eye injury may result WARNING UV emitted from this...

Страница 23: ...5 TL HAL 50 Transmitted light stand for bioscience Axioscope 5 TL FL Transmitted light and reflected light fluorescence stand for bioscience Axioscope 5 RL Reflected light stand for material Axioscop...

Страница 24: ...TL 6 5 2 1 3 4 7 Fig 8 Main components Axioscope 5 TL 1 Binocular tube 43 2 Eyepieces 46 3 Mechanical stage 50 4 Condenser 48 5 Upper part of the stand 6 Lower part of the stand 7 Transmitted light li...

Страница 25: ...nents Axioscope 5 TL FL 1 Binocular tube 43 2 Eyepieces 46 3 Mechanical stage 50 4 Condenser 48 5 Upper part of the stand 6 Reflected light light source for fluorescence 7 Lower part of the stand 8 Tr...

Страница 26: ...ents Axioscope 5 TL RL Pol 1 Binocular tube 43 2 Eyepieces 46 3 Reflector turret 53 4 Rotary stage 50 5 Condenser 48 6 Upper part of the stand 7 Reflected light light source 148 8 Lower part of the st...

Страница 27: ...ts Axioscope 5 TL RL 1 Binocular tube 43 2 Eyepieces 46 3 Reflector turret 53 4 Mechanical stage 50 5 Condenser 48 6 Upper part of the stand 7 Reflected light light source 148 8 Lower part of the stan...

Страница 28: ...TL RL MAT 1 Binocular tube 43 2 Eyepieces 46 3 Reflector turret 53 4 Mechanical stage 50 80x60 mot with sample holder 5 Condenser 48 6 Upper part of the stand 7 Reflected light light source 148 8 Lowe...

Страница 29: ...n Axioscope 5 Vario 560 mm 2 Binocular tube 43 3 Eyepieces 46 4 Reflector turret 53 5 Stage carrier H 140 mm 6 Upper stand for Axioscope including focusing gear box 7 Reflected light light source 148...

Страница 30: ...on Components ZEISS 3 2 Controls and Functional Elements on Components 3 2 1 Axioscope 5 TL Stand 2 3 4 5 6 7 8 10 9 13 12 11 14 1 Fig 14 Controls and functional elements Axioscope 5 TL 30 Instructio...

Страница 31: ...Snap buttons left and right 6 Filter slider for transmitted light 7 Focusing drive fine adjustment left and right 8 Focusing drive coarse adjustment left and right 9 Luminous field diaphragm 10 6 posi...

Страница 32: ...l Elements on Components ZEISS 3 2 2 Axioscope 5 TL FL Stand 1 2 3 4 5 6 7 8 9 10 11 12 13 15 16 17 18 19 20 21 22 14 Fig 15 Controls and functional elements Axioscope 5 TL FL 32 Instruction Manual ZE...

Страница 33: ...e fine adjustment left and right 10 Focusing drive coarse adjustment left and right 11 Snap buttons left and right 12 6 position filter wheel operable from left and right 13 Luminous field diaphragm f...

Страница 34: ...ments on Components ZEISS 3 2 3 Axioscope 5 TL RL Pol Stand 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 1 Fig 16 Controls and functional elements Axioscope 5 TL RL Pol 34 Instruction Man...

Страница 35: ...djustment left and right 10 Focusing drive coarse adjustment left and right 11 Snap buttons left and right 12 6 position filter wheel operable from left and right 13 Luminous field diaphragm for trans...

Страница 36: ...l Elements on Components ZEISS 3 2 4 Axioscope 5 TL RL Stand 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 Fig 17 Controls and functional elements Axioscope 5 TL RL 36 Instruction Manual ZE...

Страница 37: ...fine adjustment left and right 10 Focusing drive coarse adjustment left and right 11 Snap buttons left and right 12 6 position filter wheel operable from left and right 13 Luminous field diaphragm for...

Страница 38: ...ements on Components ZEISS 3 2 5 Axioscope 7 TL RL MAT Stand 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 Fig 18 Controls and functional elements Axioscope 7 TL RL MAT 38 Instruction Manua...

Страница 39: ...ive fine adjustment left and right 10 Focusing drive coarse adjustment left and right 11 Snap button on the left side 12 6 position filter wheel operable from left and right 13 Luminous field diaphrag...

Страница 40: ...nction LM and switching between fluorescence channels 4 Luminous field diaphragm for reflected light 5 Focusing drive fine adjustment left and right 6 Focusing drive coarse adjustment left and right 7...

Страница 41: ...L illumination alternately on off The respective indicator light is continuously illu minated Second press to the RL TL button to turn off on the illumination the indicator light not affected Intensit...

Страница 42: ...or light blinks GREEN ORANGE GREEN in sequence Enabling The indicator light blinks GREEN GREEN GREEN in sequence Stage control button on the left Axio scope 7 Short press Switches between XY stage con...

Страница 43: ...oscopic image can be sent to a connected camera Position The binocular photo tubes are mounted on the top of the stand Function The pupil distance and the viewing height can be adjusted with the aid o...

Страница 44: ...ular section The following features and controls are available reversed image camera port with toggle graduation 100 0 0 100 viewing angle 30 eyepiece shutter field of view 23 mm 4 7 5 3 2 1 6 Fig 21...

Страница 45: ...il distance and the viewing height can be adjusted with the aid of the binocular section The following features and controls are available upright image camera port with toggle graduation 100 0 0 100...

Страница 46: ...ective vision 3 Diopter scale to facilitate finding the correct setting 3 2 9 2 Eyepieces with Eyepiece Reticles Purpose The eyepieces with eyepiece reticles serve to observe the microscopic image in...

Страница 47: ...beam path Position The nosepiece is mounted on the upper part of the stand The following features and controls are available nosepiece with M27 mounting thread for six objectives one objective positi...

Страница 48: ...right op tional knurled screw 3 Orientation groove 4 Main spring 5 Fastening screw for height stop 3 2 12 Condensers 3 2 12 1 Condenser 0 9 1 25 BF Purpose Condensers are used to optimize the transmit...

Страница 49: ...The condenser is mounted on the condenser carrier of the stand 3 1 7 5 6 4 2 Fig 28 Condenser 0 9 1 25 BF DF Ph1 Ph2 Ph3 with modulator disk 1 Lever for setting the aperture di aphragm 2 Front lens 3...

Страница 50: ...X and Y direc tion The adjustment range can be read off the respective vernier scale The following features and controls are available rackless stage coaxial drives in X and Y adjustment on the right...

Страница 51: ...y Function The sample is fixed on the stage by means of insert plates 160x116 or mounting frames for two sample sliders 76x26 that are inserted into the stage supporting surface The sample is position...

Страница 52: ...range can be read off the respective vernier scale The following features and controls are available optionally equipped with attachable sample guide for use of standard slides 45x25 mm and 75x25 mm 3...

Страница 53: ...nation data of the reflector module and pasted on the corresponding field 3 Knurled ring to swivel the desired re flector module into the beam path 3 2 14 2 Reflector Slider with Two Coded Positions...

Страница 54: ...diffracted and scattered at the prepara tion details are of essential importance According to ABBE the larger the indirect beam compo nents are the more true to the object the microscopic image is The...

Страница 55: ...oring sample locations at a short distance and experience different path differences there due to differences in refractive index and sample thickness Both beams are then combined in a second birefrin...

Страница 56: ...illation di rection n pointing strongly to northeast southwest The changes in color are based on optical interference It is necessary to compare the interference colors path differences in both diagon...

Страница 57: ...northeast southwest di rection the path difference of the sample e g gray white 150 nm and the path difference of the lambda compensator red 550 nm add up This results in a color change of the sample...

Страница 58: ...occurrence of the color change effect may be the evaluation in two sample positions rotated at an angle of 90 from one another For this purpose rotate the centered stage by 2 click stops The suitable...

Страница 59: ...BE the larger the indirect beam compo nents are the more true to the object the microscopic image is The cone of light emerging from the reflected light light source is reflected on a color neutral be...

Страница 60: ...550 nm When interference fil ters are used it is important to apply the focal point of their wave lengths The measured path difference depends on the aperture and increases with the illumination aper...

Страница 61: ...rve as approximate values since the phase jumps depend on the layerthickness and the angle of incidence of the light An ac curate determination of the layer thickness is possible only when the complet...

Страница 62: ...flective cap helps eliminate the reflections which are inevitable when working with objectives with very low magnification A sample is bireflectant when the sample details show differences in brightne...

Страница 63: ...aging 2 Take the microscope all assemblies and accessories out of the packaging 3 Check them for completeness as per delivery note 4 Check all parts for damaging 5 Place the microscope on a vibration...

Страница 64: ...3 5 Fig 37 Installing the upper stand part on the stand column 1 Stand column 2 Mounting plate 3 Hex screw 6x 4 Upper stand part 5 Gear box Procedure 1 Unpack the upper stand part 4 with gear box 5 an...

Страница 65: ...Remove the dust cap 1 from the dovetail ring mount on the stand side 3 Remove the dust cap 4 from the underside of the tube 3 4 Hold the tube at an angle insert it with the dovetail ring into the stan...

Страница 66: ...om the tube 2 Remove both eyepieces 2 from the box and insert them into the eyepiece socket of the tube to the stop NOTICE Before inserting Pol eyepieces with tubes without upright reticles the orient...

Страница 67: ...th the smallest magnification factor set up clockwise in nosepiece position 1 5 Make sure it engages properly in the nosepiece s thread 6 Instead of an objective the sample marker 5 with an adapter W0...

Страница 68: ...y into the hole 2 2 Turn the locking screw counterclockwise 3 Remove the cover cap 1 to the front 4 Push the reflector turret 3 with the reflector modules P C e g reflector turret with 6 coded positio...

Страница 69: ...0 mm Procedure 1 Place the stage 1 on the stage carrier 7 so that the threaded holes 2 on the bot tom of the stage are positioned above the stage carrier openings 3 and hold the stage 2 Insert four f...

Страница 70: ...g pin 3 Press the stage forward against the spring loaded pin and lower its back side into the stage carrier 4 4 Tighten the screw cap Proceed in the reverse order for removal 4 7 3 Assembling the Mot...

Страница 71: ...of the stage carrier 4 Align the stage in XY direction 5 Tighten the screws Use a 3 mm hex key 4 6 Install the stage carrier together with the stage on the stand 7 Plug the power cable connectors into...

Страница 72: ...ier along the guide upward until it engages in the shoulder screw 7 Tighten the wing screw 8 Check to ensure that the stage carrier is accurately positioned 4 9 Assembling the Condenser Carrier 3 2 4...

Страница 73: ...vertical adjustment push the condenser carrier down as far as it will go NOTICE Damage due to collision If using a low power system make sure that this does not come to rest on the luminous field dia...

Страница 74: ...switched off 1 2 3 5 4 6 2 Fig 45 Assembling the LED10 light source for transmitted light illumination 1 3 mm hex key 2 Clamping screw 3 LED10 light source 4 Plug 5 Socket for transmitted light illumi...

Страница 75: ...equipment protective clothing if required 4 Before installing or removing the light source always make sure it is switched off NOTICE Heat damage The HAL 100 bulb replacement tool might suffer damage...

Страница 76: ...1 from the light source housing Procedure 1 Loosen the clamping screw 3 on the illumination mount 4 of the carrier for trans mitted light illumination 2 Insert the dovetail ring of the HAL 100 2 into...

Страница 77: ...lumination 75 4 12 3 Assembling the Adjustment Tool of HBO 100 This procedure applies to the following stand type only Axioscope 5 Bio TL RL 1 4 3 2 Fig 47 Assembling the adjustment tool 1 Cover cap 2...

Страница 78: ...ot be mounted onto the standard stand When using the HAL or HBO light sources a base plate for the Axioscope 000000 2202 526 is mandatory Info For installing or replacing the HBO 103 W 2 mercury vapor...

Страница 79: ...and UV radiation Skin or eye injury can result from the exposure 4 Avoid any eye and skin exposure to the light emitting aperture of the light source 4 Avoid exposure of skin to the radiation Use suit...

Страница 80: ...clamping screw 4 Connect the LED light source plug 5 to the stand socket 4 5 Tighten the fixing screws on the plug Proceed in the reverse order for removal 4 12 6 Assembling the HXP 120 V Light Sourc...

Страница 81: ...operation and kept for further use The chapter Safety was read and understood The operator is acquainted with the general Windows based programs If required Basic training and safety briefing were suc...

Страница 82: ...u see only one round image while looking through the two eyepieces Procedure 1 Set the interpupillary distance by rotating the eyepiece tubes symmetrically toward or away from one another 2 Set the vi...

Страница 83: ...ond eye with the focusable eye lens of the second eyepiece 9 Both microscopic images including the eyepiece reticle are thus in focus From this point use only the focusing drive for any subsequent foc...

Страница 84: ...when the sample is lifted out Carefully raise the condenser by a small amount without lifting out the sample 6 Tighten the set screw of the height stop 5 3 5 Adjusting the Height Stop on the Focusing...

Страница 85: ...on Prerequisite The microscope is operational Procedure 1 Press one of the Snap button and the Inten sity LM knob simultaneously for at least 1 5 seconds The indicator light blinks in the following se...

Страница 86: ...r combination is saved When using LED as light source the LED is switched off for 300 ms This is visible through the eyepieces and serves as an indicator for the user 4 Switch to the second objective...

Страница 87: ...manent mode switch 5 4 Setting Up for Transmitted Light Techniques 5 4 1 Setting Up for Transmitted Light Brightfield Microscopy Using the K HLER Method High contrast sample slide Every microscope exc...

Страница 88: ...ob on the microscope stand 3 Insert the high contrast sample into the sample holder of the stage 4 Set position H or B brightfield when using condensers with a turret modulator disk and knurled ring 5...

Страница 89: ...knob for vertical adjustment to the upper stop 7 Swivel in the 10x objective on the nosepiece 8 Focus the sample 9 Close the luminous field diaphragm until it is visible even if not in focus in the fi...

Страница 90: ...d of view D D 13 Remove an eyepiece from the binocular tube to adjust the aperture diaphragm contrast 14 Look into the tube with the naked eye 15 Set the aperture diaphragm with the adjusting lever to...

Страница 91: ...adjustments must be repeated With objectives 10x the front lens of the condenser if swivelable must be swivelled out of the beam path and the aperture diaphragm completely opened For better contrast w...

Страница 92: ...denser for transmitted light darkfield microscopy is installed 73 The illumination is adjusted for transmitted light brightfield microscopy 87 Procedure 1 Set the modulator disk to position D or DF da...

Страница 93: ...darkfield diaphragm in the objective exit pupil The objective exit pupil must appear homogeneously dark 12 If necessary center the darkfield diaphragm 13 If required remove the auxiliary microscope 14...

Страница 94: ...iaphragm is easier to identify 7 Close the luminous field diaphragm until the end stop 8 Lower condenser until the edge of the field diaphragm appears sharp luminous field di aphragm focus level An in...

Страница 95: ...12 Swivel in an immersion oil objective 13 Focus the sample 14 Close the luminous field diaphragm until the end stop 15 Lower the condenser until the edge of the field diaphragm appears sharp luminou...

Страница 96: ...ne eyepiece 66 with an auxiliary microscope 4 With the adjusting fixture on the auxiliary microscope focus the annular phase diaphragm and the phase ring in the objective exit pupil 5 Check the center...

Страница 97: ...ctor insert 3 Objective on the nosepiece 4 Condenser with DIC prism 5 Knurled screw for optimal contrast 6 DIC slider 7 Compensator Prerequisite The microscope is operational Objective equipped with D...

Страница 98: ...0x or 4 5 mm slit diaphragm for PlasDIC in all other cases is installed One of the following objectives is installed 67 A Plan 10x 20x 40x LD A Plan 20x 32x 40x LD Plan Neofluar 20x 40x 63x DIC slider...

Страница 99: ...The Pol objectives are centered 182 5 4 8 1 Detecting Birefringence For more information about the method see chapter Detecting Birefringence 55 2 1 3 1 Rotary stage 2 Polarizer 3 Analyzer Procedure 1...

Страница 100: ...The fiber is parallel to one of the two lines in the cross hair orientation 11 Turn the rotary stage by approx 45 until the longitudinal axis of the fiber is pointing in northeast southwest direction...

Страница 101: ...the lower part 2 of the circular polarizer into the light path up to the click stop 3 Swing in the analyzer module on the reflector turret 1 or insert the analyzer slider into the intermediate plate 4...

Страница 102: ...icroscopy are available The achromatic aplanatic 0 9 H Pol condenser or 0 9 Pol condenser is installed The D polarizer rotatable or fixed is installed Analyzer slider or D Pol analyzer module in the r...

Страница 103: ...r 14 140 or the eyepiece reticle to the vibration direction of the polarizer using the adjustment aid 100 Remove the Pol adjustment aid 5 Move a selected crystal to the center of the reticle 6 Swivel...

Страница 104: ...py 87 Procedure 1 Place the sample on the stage 2 Focus the sample 3 Swivel the polarizer 4 Swivel the D Pol analyzer module into the light path 5 If you do not use the Pol binocular photo tube carry...

Страница 105: ...led The microscope is operational for reflected light microscopy The microscope is adapted 82 to the user Procedure 1 If required push the RL button for reflected light illumination 2 Adjust the image...

Страница 106: ...mple 7 Turn the knurled button of the aperture di aphragm to a medium position 8 Adjust the knurled knob on the field diaphragm so that the field diaphragm becomes visible in the field of view A A 9 U...

Страница 107: ...x 2 3 4 5 of the diameter of the objective exit pupil E In most cases this aperture gives the best con trast at almost full resolution and is thus the best compromise for the human eye E 14 Replace th...

Страница 108: ...ofluar HD Epiplan HD The microscope is operational The illumination is adjusted for reflected light brightfield microscopy 105 The field diaphragm image should lie just barely outside of the edge of t...

Страница 109: ...table or the rotary stage Pol is installed 69 The microscope is operational DIC reflector module is installed A suitable objective for DIC is installed 67 e g EC Epiplan Neofluar Epiplan with the addi...

Страница 110: ...er Reflected Light DIC and C DIC Microscopy 59 Prerequisite A reflected light light source is installed 77 The microscope is operational C DIC reflector module is installed A suitable objective for DI...

Страница 111: ...re information about the method see chapter Reflected Light TIC Microscopy 60 Prerequisite The HAL 100 light source is installed 77 The microscope is operational A suitable objective for DIC is instal...

Страница 112: ...of view Use the set ting screw 3 7 To choose the structure to be measured turn the setting wheel 2 on the TIC slider until the interference stripes are vertical to the direc tion in which the sample...

Страница 113: ...ary stage Pol 4 Analyzer slider 5 Polarizer slider Prerequisite A reflected light light source is installed 77 The rotary stage Pol is installed 69 The microscope is operational The DIC reflector modu...

Страница 114: ...yzer is turned off 5 5 7 Setting Up for Reflected Light Fluorescence Microscopy The present section applies to the following microscope type Axioscope 5 TL FL 430035 9061 000 For more information abou...

Страница 115: ...nosepiece 2 Slide the fluorescence protection shield into the 6x20 mm slot 3 Swivel in the EC Plan Neofluar objective on the nosepiece 5 4 Initially set transmitted light illumination by pushing the T...

Страница 116: ...Using both centering screws 2 center the field diaphragm on the edge of the field of view 19 Open the field diaphragm enough to make it just disappear behind the edge of the field of view or if you ar...

Страница 117: ...ies and property damage and voids all rights to warranty claims Only original spare parts from ZEISS may be used DANGER Electric shock due to live parts When the microscope is still switched on coming...

Страница 118: ...light source Replace the LED modules If the travel range in X direc tion will gradually become smaller Mechanical stage Recovering the stage travel range 127 Tab 6 Maintenance Plan 6 3 Maintenance Wo...

Страница 119: ...nd 15 vol isopropyl alcohol IPA Lint free cloth Procedure 1 Moisten a cotton swab or a clean cloth with an optical cleaning solution if necessary 2 Wipe optical surfaces in a circular motion to wards...

Страница 120: ...oled down for about 15 minutes Procedure 1 Remove the HAL 50 halogen light source 2 from the back of the stand 1 1 2 2 Put it down with the open side facing up 3 Remove the used lamp 3 in upward direc...

Страница 121: ...e clothing if required 4 Before installing or removing the light source always make sure it is switched off CAUTION Burning hazard due to hot light sources Light sources can become hot during processi...

Страница 122: ...o center the bulb briefly press the spring levers once more 7 NOTICE Do not leave the bulb replacement tool inside the light source Insert the lamp carrier into the Hal 100 housing 1 and slide it in u...

Страница 123: ...down Procedure 1 NOTICE Make sure the light source does not fall when unfixing it Hold the light source and loosen the clamping screw at the light source mount of the stand 2 Remove the light source...

Страница 124: ...le is connected to the corresponding socket at the external power supply All filters are removed from the beam path Procedure 1 Swivel in an objective with a magnification of 40x or lower 2 Bring a fr...

Страница 125: ...source is switched on and has reached operational temperature If the FL attenuator is in the reflected light beam path make sure that it is set to 100 trans mission Procedure 1 Pull the adjustment to...

Страница 126: ...ling or removing the light source always make sure it is switched off For more information about the usability of LED modules for Colibri 3 see Usability of LED Mod ules for the Colibri 3 LED Light So...

Страница 127: ...required replace the LED modules of LED slots 2 3 and 4 in the same way 10 Re mount the housing 6 3 6 1 Usability of LED Modules for the Colibri 3 LED Light Source Position Slot 1 Slot 2 Slot 3 Slot...

Страница 128: ...holder 3 Fuse compartment Parts and Tools 2x Fuse type T3 15A H 250V Prerequisite The microscope is switched off and disconnected from the mains Procedure 1 If the fuses fail first check the cause an...

Страница 129: ...mains 2 1 3 1 Fuse 2 Fuse holder 3 Fuse compartment Parts and Tools 2x Fuse type T5 0 A H 250V 5x20 mm Prerequisite The microscope is switched off and disconnected from the mains Procedure 1 If the fu...

Страница 130: ...is not fully engaged in its locking position Engage the nosepiece with the objective in its locking posi tion Condenser is not adjusted cor rectly Adjust the condenser correctly adjustment centering s...

Страница 131: ...correct thickness of the frame glass Dirt or dust on the optical sur faces of objectives eyepieces condensers or filters Clean the soiled optical com ponent Parfocal performance not good on Axioscope...

Страница 132: ...orrectly on the mechanical stage Insert and fix the sample cor rectly in the sample holder Distinct focus differences when changing the ob jective Focusable eyepieces are not adjusted correctly Adjust...

Страница 133: ...mitted Light Brightfield Microscopy Using the K HLER Method 87 Dirt of dust on optical surfaces of objectives eyepieces con densers filters or samples Clean the optical surfaces of the soiled componen...

Страница 134: ...placing the 12 V 50 W Halogen lamp of the HAL 50 Halogen Illuminator 120 7 1 Resetting the Microscope to the Factory Settings NOTICE Please use this function with caution as it will reset all existing...

Страница 135: ...The boxes must be secured during transport Avoid rocking the boxes back and forth Note the weight information on the package and on the shipping document Where possible the original packaging must be...

Страница 136: ...d The customer is responsible for decontamination Info Detailed information on disposal and recycling is available from your ZEISS Sales Service Part ner 8 4 Decontamination A decontamination statemen...

Страница 137: ...ed near radiators or windows with direct sunlight Compliance with the installation requirements of the microscope and the availability of the re quested supplies is the responsibility of the customer...

Страница 138: ...g earth point at all times not applicable for Axioscope 5 Vario Fuses in the Axioscope 5 7 stand 2x T 3 15 A H 5x20 mm in compliance with IEC 127 Fuses in the HBO 100 W power supply unit T 2 0 A H 5x2...

Страница 139: ...None 449 485 Binocular photo tube 30 23 100 100 30 None 449 485 Binocular photo tube 20 23 100 100 20 None 442 481 Binocular ergo tube 15 23 50 50 tele scopic height up right image 15 height telescopi...

Страница 140: ...5 EU RoHS the microscope and its accessories have been clas sified as instrument category 9 Monitoring and control instruments including industrial monitor ing and control instruments They also fall u...

Страница 141: ...bjectives for your application depends on various factors More detailed information on available and recommended objectives can be found at https www micro shop zeiss com de de shop objec tives or ask...

Страница 142: ...anical stage 75x50 R with special surface for high load ca pacity Mechanical stage 75x50 R for reflected light Sample holders The following sample holders are available Specimen holder for reflected l...

Страница 143: ...100 0 0 100 reversed image Binocular phototube 20 23 100 0 0 100 upright image Binocular phototube Pol 20 23 100 0 0 100 upright image Reflector inserts The following reflector inserts are available...

Страница 144: ...he top of the stand Function The pupil distance and the viewing height can be adjusted with the aid of the binocular section The following features and controls are available Optionally with upright o...

Страница 145: ...the viewing height can be adjusted with the aid of the binocular section The following features and controls are available upright image camera port with toggle graduation 100 0 0 100 viewing angle 20...

Страница 146: ...ocular section The following features and controls are available upright image camera port with toggle graduation 100 0 0 100 viewing angle 20 field of view 23 mm usable 22 mm vertical adjustment of 4...

Страница 147: ...e adjusted with the aid of the binocular section Depending on the design the following features and controls are available upright image camera port with fixed light graduation 50 50 viewing angle 15...

Страница 148: ...the transmitted light process Position The HAL 100 is installed depending of the light path reflected or transmitted 1 3 4 5 6 2 Fig 62 HAL 100 1 Lamp housing 2 Dovetail ring 3 Vertical adjusting scr...

Страница 149: ...placed beside the microscope 1 2 3 4 5 6 7 8 Fig 63 External power supply for HAL 100 front and rear side 1 Power switch 2 Control knob for illumination intensity 3 Indicator light Extern 4 Toggle sw...

Страница 150: ...ight source can damage the gray filter for reflected light during prolonged use 4 Use an attenuator instead of a gray filter to change the light intensity in the reflected light path Info For installi...

Страница 151: ...ovetail ring into the illumination mount 3 Fasten the clamping screw 4 Insert the multi pin plug of the HBO 100 into the device connector 4 on the PSU 5 5 4 6 7 8 5 Fasten the connector s coupling rin...

Страница 152: ...n instrument is switched on 2 Indicator lights LAMP lights when the lamp has been ignited and is lighting TEMP lights when the tempera ture inside the transformer is within the permissible range SAFET...

Страница 153: ...ry tightly in the eyepiece groove so you may need a blunt sample wooden stick to prod them off 10 4 Analyzer Sliders 10 4 1 Analyzer Slider TL RL Fixed Purpose The analyzer slider is used to set the p...

Страница 154: ...ion contrast technique Position The analyzer slider is inserted into the 12x46 slot of the intermediate plate for analyzer slider 12x46 mounted between stand and tube 4 3 1 2 Fig 68 Analyzer slider TL...

Страница 155: ...reflected light Function One stop slider is required to function as a luminous field diaphragm F and the other to function as the aperture diaphragm A Info When using fluorescent light an FL attenuato...

Страница 156: ...he adjustment range can be read off the respective vernier scale The following features and controls are available 240 rotatable with clamping function coaxial drives in X and Y adjustment on the righ...

Страница 157: ...for transmitted light brightfield microscopy 87 Procedure 1 Loosen the clamping screw 2 of the stage 2 Loosen the screw cap 4 of the stage carriers 3 Turn the stage until the maximum shift of the sam...

Страница 158: ...ed on the stage carrier of the stand Function The sample is fixed on the stage by means of the clamping device The following features and controls are available 360 rotation with lock click stop every...

Страница 159: ...of the spring box with approx 3 turns 2 Put the rotary 1 stage with the dovetail ring notch onto the spring loaded pin 4 The knurled screw 3 must point to the front right 3 Press the stage forward aga...

Страница 160: ...is installed in the stage carrier The K HLER illumination 87 is adjusted A high contrast sample and an eyepiece with a crossline reticle are available Procedure 1 Loosen the knurled screw for locking...

Страница 161: ...stages are equipped with additional sleeves for an even more sensitive adjustment of the sample position These sleeves can be removed when a faster sample movement is important Procedure 1 Loosen bot...

Страница 162: ...clockwise until the desired freedom of movement has been achieved Small friction adjustment clockwise Large friction adjustment counter clockwise It should not be shifted more than one revolution Y dr...

Страница 163: ...the X coaxial knurled knob 4 down ward and the Y coaxial knurled knob 2 up ward 1 2 4 3 2 Hold the X coaxial knurled knob 4 and turn the light colored knurled ring above it 3 to the right increased s...

Страница 164: ...pring clip left right 5 Upper spring clip left right Procedure 1 Remove the reflector insert 68 1 from the upper part of the stand 2 Put the reflector insert aside with the top side facing down 3 NOTI...

Страница 165: ...m splitter 8 Beam splitter Please note the following orientation rules Emission filters 5 with a direction indicating arrow on their circumference must be in stalled with the arrow pointing to the out...

Страница 166: ...tweezers to carefully grab the filter at its circumference 4 Place the filter on the reflector module s respective position Observe the correct orienta tion 13 5 Screw on the retaining ring 10 8 3 Ch...

Страница 167: ...m splitter 7 lies in front of you 4 Remove the beam splitter and the spring loaded frame 8 5 Remove the beam splitter from the spring loaded frame 6 Use tweezers to pick up the new beam splitter 7 Pos...

Страница 168: ...e clamping screw 2 of the condenser s dial segment 1 and pull out the dial segment 2 Slide the modulator disk 5 with its two pronged forked opening pointing forward into the condenser opening 4 3 Make...

Страница 169: ...lator disk is removed 168 Procedure 1 Turn the PhC diaphragm that needs to be replaced into the free aperture of the modulator disk 2 Screw the modulator disk s centering screws 7 back until they stop...

Страница 170: ...m must be deactivated For this turn the center ing screws of the modulator disk counterclockwise to the stop Now the aperture diaphragm can be closed for DIC contrast techniques 10 10 Assembling the S...

Страница 171: ...the Cable Connections 5 6 7 8 Fig 81 Disconnecting the cable connections 5 Cable connector of the main control board 6 Cable connector of the turret sensor RL LED interface 7 Cable connector of the ma...

Страница 172: ...0 10 4 Assembling the Sample Space Extension 10 11 7 12 5 Fig 83 Installing the sample space extension 5 Cable connector of the main control board 7 Cable connector of the main control board 10 Spacer...

Страница 173: ...Cable connector of the main control board 7 Cable connector of the main control board 9 Fixing screw 6x 10 Spacer bolt 6x 12 Lateral opening Procedure 1 Carefully route the cables 5 and 7 from below...

Страница 174: ...ontrol board 7 of the lower stand part and the nosepiece sensor of the upper stand part 8 2 Establish the cable connection between the main control board 5 and the turret sen sor RL LED interface 6 3...

Страница 175: ...of the polarizer for swivelling in out 3 Holding pin 4 Locking clamp 10 11 2 Polarizer D 90 Rotatable Removable Purpose The polarizer for transmitted light is used to polarize the light of the transmi...

Страница 176: ...7 Lambda plate rotatable by 90 8 Polarizer 10 11 4 Polarizer Rotatable with Color Filter Carrier Purpose The polarizer for transmitted light is used to polarize the light of the transmitted light sou...

Страница 177: ...er 8 Handle of the lower part of the circular polarizer for swivelling in out 9 Adjustment slit 2x 10 11 6 Color Filter Carrier 3x for Filter d 32mm Purpose With the help of the color filter carrier o...

Страница 178: ...rrier 1 2 Insert the holding pin 4 into the front threaded opening at the left below the condenser carrier 3 Tighten the holding pin with the adjusting lever 3 4 Screw the locking pin 2 with the adjus...

Страница 179: ...1 3 1 2 Fig 93 Low power system for objectives 2 5x 4x 1 Centering screw 2x 2 Locking pin 3 Handle of the low power system for swivelling in out 4 Holding pin 5 Locking clamp Instruction Manual ZEISS...

Страница 180: ...e underside of the condenser carrier 1 and screw the holding pin 5 of the low power system with the angled adjusting lever 3 into the front left threaded hole below the condenser carrier as far as it...

Страница 181: ...l object guide 1 introducing the two cylindrical pins on its underside into the respective holes 6 4 If required turn the control wheel 5 until the clamping screw becomes visible in the mounting hole...

Страница 182: ...illumination 87 is adjusted The rotary stage is centered 160 using the non centerable objective mount A high contrast sample and an eyepiece with crossline reticle are available Procedure 1 Turn the n...

Страница 183: ...ig 97 Axiocam 202 mono 208 color 1 OSD menu button 2 Image video capture button 3 Camera factory reset button 4 Status LED 5 HDMI port for image data transfer to a monitor TV or projector 6 Gigabit Et...

Страница 184: ...pter 2 on the Axiocam 1 2 Attach the Axiocam with the adapter to the camera port 4 of the tube 3 Orient the camera to the stand and fix it in position by tightening the ring nut 3 4 Connect the camera...

Страница 185: ...an be connected via the USB slot at the back of the camera for storing data Then images are recorded and saved to the USB drive Functions of the microscope stand such as the Light Manager and encoding...

Страница 186: ...ger encoding and image enhancement are automatically launched Live images can be viewed on the monitor display and advanced features are available in the on screen display OSD When the microscope is o...

Страница 187: ...the Light Manager encoding ECO mode and image enhancement are automat ically launched When a monitor is connected live images can be viewed on the monitor display Live images can also be viewed on PC...

Страница 188: ...Wi Fi Functions such as the Light Manager encoding ECO mode and image enhancement are automat ically launched When a monitor is connected live images can be viewed on the monitor display Live images...

Страница 189: ...ht Manager encoding ECO mode and image enhancement are automat ically launched When a monitor is connected live images can be viewed on the monitor display Live images can also be viewed on a PC or Su...

Страница 190: ...microscope stand via USB cables at the same time Functions such as the Light Manager encoding and ECO mode are automatically launched Live images can also be viewed on the workstation and basic featu...

Страница 191: ...Lever for switching the front lens in out left right 3 Knurled ring for adjusting the position of the 5 positions turret disk 4 Sliding control for setting the aperture diaphragm 5 Dovetail ring mount...

Страница 192: ...enser carrier until the maximum vertical ad justing range is usable 5 Using the knurled knob 8 for vertical adjustment push the condenser carrier down as far as it will go NOTICE If using a low power...

Страница 193: ...l of the objective appears homogeneously dark 5 Insert the eyepiece 10 14 3 Centering the Annular Phase Diaphragm of the Condenser Parts and Tools 2 x Hex key 1 5 mm Prerequisite A suitable condenser...

Страница 194: ...d 2 1 3 4 Fig 106 Installing the base plate on the stand 1 Stand 2 Rubber foot 4x 3 Base plate 4 hexagonal socket screw 4x Procedure 1 Remove the four rubber feet 2 from the bottom of the stand 1 2 Al...

Страница 195: ...lens of the tube accessible from the bottom Use the included ring tool 4 Screw the tube lens included with the intermediate plate into the binocular tube 5 Insert the intermediate plate 5 with the dov...

Страница 196: ...fication changer 1 Magnification changer 2 Retainer nut 3 Camera port of the photo tube Prerequisite The microscope is switched off Procedure 1 Remove the camera camera adapter 184 or the dust protect...

Страница 197: ...n 3 Screw 3x 4 Luminous field diaphragm socket Prerequisite The stage carrier is removed 71 Procedure 1 Unscrew three screws 3 of the luminous field diaphragm socket 4 2 Remove the socket from the sta...

Страница 198: ...Revi sion Date of Issue Introduced Modifications 13 04 2022 Implementation of revision history Adaptation to Regulation EU 2017 746 IVDR Tab 7 Revision History 198 Instruction Manual ZEISS Axioscope 5...

Страница 199: ...luorescing object Hz Hertz a measure for frequency P C Push and Click PCBA Printed Circuit Board Assembly PlasDIC Differential Interference Contrast for Plas tic Receptacles PSU Power supply unit RL R...

Страница 200: ...ISS Sales Service Partner The Sales Service Partner is generally in the field for customer support in a re gional area and or a clearly defined cus tomer group ZEISS service representative Specially t...

Страница 201: ...ht stop 84 assembling 72 Coniscopic observation 59 Connecting to the mains 80 Conoscopic 102 Contamination 136 D Darkfield 54 59 92 94 95 108 Darkfield condenser assembling 73 Decontamination 136 Annu...

Страница 202: ...gth 161 Mechanical stage motorized installing 70 Mechanical stage rotatable centering 157 Modulator disk installing 168 N Nosepiece 47 67 O Object guide installing 181 Objective 47 67 centering 182 Op...

Страница 203: ...C 60 111 Training 16 Transmitted light polarization 59 conoscopic 102 Transmitted light brightfield 54 88 darkfield 54 92 94 95 DIC 55 98 phase contrast 55 96 PlasDIC 55 98 polarization 55 99 Transpor...

Страница 204: ...GmbH Carl Zeiss Promenade 10 07745 Jena Germany phone 49 1803 33 63 34 fax 49 3641 64 3439 info microscopy de zeiss com www zeiss com microscopy Instruction Manual ZEISS Axioscope 5 Axioscope 5 7 MAT...

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