Bio-Tek ELx405, Руководство оператора

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| Chapter 3: Operation
Define Wash METHOD Program Options
Option & Description Models Action/Comments
Number of Cycles
Specify 1 to 10 complete
wash cycles.
All A complete wash cycle represents an
aspirate and dispense sequence, which
may or may not end with a final aspiration
of the liquid from the wells.
Note: If Bottom Wash is enabled, an
additional aspirate and dispense is
performed using unique bottom-washing
dispense parameters.
Wash Format
Specify how the wells
should be processed
(384-well plate).
Select,
Select CW,
HT/96-tube Only appears if Plate Type is 384.
Plate format applies each cycle to the
entire plate before it starts the next cycle.
Sector format applies each cycle to one
quarter of the plate before it moves to the
next quarter (sector).
Note: For higher throughput of the HT
with 384-well plates, use the 192-tube
manifold.
Soak/Shake?
Specify soaking and/or
shaking.
All Choose YES to soak and/or shake the
wash buffer in the microwells after the
buffer has been dispensed.
Soak Duration
Specify the time to allow
the wash buffer to remain
in the wells before
aspiration.
All The duration range is from 0 to 600
seconds.
A soak begins after the wash buffer is
dispensed to the wells. In some
chemistries it enhances washing by
allowing unbound material to diffuse into
the wash buffer.
When washing wells in a plate format, use
a soak that lasts as long as it takes to
process one wash cycle of all wells.
Shake Before Soak?
Specify whether to
agitate the microwells
back and forth linearly
along the carrier track to
aid in dispersal.
All Select YES or NO .
Before the microwells begin shaking, the
manifold raises so that the dispense and
aspirate tubes clear the microwells, and
the carrier returns to its home position.
Shake Duration
Specify the length of time
for microwell agitation.
All The duration range is from 1 to 600
seconds.
Shake Intensity
Specify the intensity of
microwell shaking.
All The options range from 1 to 5, where
1 = Least Intense, and 5 = Most Intense.
The washer display shows the
corresponding cycles/sec value for each
option.
Bio-Tek Instruments, Inc.