Bio-Tek ELx405, Руководство оператора

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Liquid Tests
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153
Dispense Precision Test (96)
This test is designed for Single/96 and Dual/96 manifolds. See page
160 if you’re looking for the Dispense Precision Test for the Dual/192
manifold.
See page 148 for a list of required materials.
Without the Valve Module
1. Fill a supply bottle with two liters of deionized water. Run the Prime
program
P_DAY_RINSE two or three times to prime the fluid lines and
manifold.
2. Fill a supply bottle with 1200 ml of Solution #3 .
3.
Run
P_DAY_RINSE
to prime the washer with the solution.
4. Place a clean, dry 96-well microplate on the washer and run the Dispense
program
accuracy_qc_test . This program dispenses 300
µ
l of solution to
each well of the plate. It does not evacuate the solution.
5. When the program is finished, carefully remove the plate.
If you are using one of Bio-Tek’s keypad-based readers, such as the
ELx800
 or ELx808  , ensure that the reader is not running in Rapid
mode. To check the setting, select
UTIL READ
and cycle through the
options until
READ IN RAPID MODE?
appears. Set it to NO .
6.
Read the plate in an optical reader (blank on air), using the
dual-wavelength method (630 nm - 450 nm), then print or export the
results.
7. Using the
Dispense Precision Test Worksheet
on page 165, perform data
reduction:
a. Calculate the
Standard Deviation .
Tip: If you have a spreadsheet software program, enter/export all 96
values into a spreadsheet and apply your program’s Standard
Deviation function (e.g., Microsoft Excel’s STDEV).
b. Calculate the sum of the OD values for all 96 wells, then divide by 96
to determine the Mean OD for the plate.
c. Calculate the %CV : (Standard Deviation / Mean OD) * 100.
The %CV should be
<=
4.0 .
ELx405 Operator's Guide