Bio-Tek ELx405, Руководство оператора

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| Chapter 5: Performance Verification/Qualification Tests
c. Calculate the %CV: (Standard Deviation / Mean OD) * 100.
The %CV should be
<=
4.0 .
8. If the %CV is greater than 4.0, one or more dispense tubes may need to be
cleaned. Run AUTOCLEAN (Ultrasonic Advantage™ models only), and/or
remove the manifold and use the stylus to clean the dispense tube(s) giving
lower-than-average absorbance readings (all models). When finished, re-
prime the washer and retry the test.
9.
When finished, prime with deionized water to flush out the dye solution.
Annual Procedure for All Valves
Perform this test during the annual OQ only.
The routine test for Valve A must pass before the annual test for
Valves A-D can be performed.
1. Empty the waste bottle now, and then as needed throughout this
procedure.
2. Fill each of the supply bottles connected to Valves A, B, C, and D with
three liters (3000 ml) of deionized water.
Repeat the following steps for each valve:
3. Run the Maintenance program DAY_RINSE two or three times to prime
the fluid lines, manifold, and the valve being tested.
4. Edit the Dispense program 192accuracy_qc to use the Valve currently
being tested ( DEFINE EDIT DISP 192accuracy_qc ).
5. Place a clean, dry 384-well microplate on the balance and zero the balance.
6. Place the microplate on the washer and run the Dispense program
192accuracy_qc . This program dispenses 80 µ l of water to each well of
the plate. It does not evacuate the wells.
7. When the program is finished, carefully remove the plate and weigh it. This
is the
Total Dispense Weight in grams.
The weight should be 30.72 grams, ± 10% (between 27.65 g and
33.79 g).
If the weight falls outside this range, contact Bio-Tek.
Bio-Tek Instruments, Inc.