Bio-Tek ELx405, Руководство оператора

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Chapter 4: Preventive Maintenance
Alternate Decontamination Procedure for Tubing and Manifold
If you are unable to run the DECONTAMINATION program due to a system failure,
perform the following alternate decontamination procedure to disinfect the
internal tubing and manifold.
Caution! Be sure to check the percent NaClO of the bleach
you are using; this information is printed on the side of the
bottle. Commercial bleach is typically 10% NaClO; if this is the
case, prepare a 1:20 dilution. Household bleach is typically 5%
NaClO; if this is the case, prepare a 1:10 dilution.
1. Turn the washer off and disconnect the power cord.
2. Remove the mist shield, if it is attached:
Using your fingers, loosen the two thumbscrews on the shield ( use no
tools ).
Lift the shield off the two posts.
Refer to
Decon Procedure for External Surfaces of the Instrument
on page 129 for decontamination of the mist shield.
Use extra care when removing the Select , HT , and Select CW
dual manifolds. Hold the two manifolds (and end plates) together
as a single unit when removing or replacing.
3. Using the 9/64” (3.57 mm) hex wrench, remove the screws, washers, and
springs that hold the manifold(s) in place (see
Figures 25
,
26
, and
27
).
Remove the manifold(s) and end plates.
4. Prepare an aqueous solution of 0.5% sodium hypochlorite (bleach). As an
alternative, 70% isopropyl alcohol (or ethanol) may be used if the effects of
bleach are a concern.
Isopropyl alcohol is not recommended for removing proteins
(such as bovine serum albumin).
5. Soak the tubing and manifold in the bleach or alcohol solution.
6. Wait 20 minutes. Rinse the tubing and manifold with DI or distilled water.
7. Use a clean, dry cloth to dry all wet surfaces of the tubes and manifold.
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