Bio-Tek ELx405, Руководство оператора

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| Chapter 3: Operation
Define Wash ASPIRATE (ASPIR) Program Options, Cont’d
Option & Description Models Action/Comments
All A crosswise aspiration is a two-step
aspiration. The wells are
first aspirated
at the Horizontal Aspirate Position.
The aspirating tubes raise a fixed number
of steps and then do a second (crosswise)
aspiration at the Crosswise Horizontal
Position.
Crosswise Aspir?
Specify whether to
perform a Crosswise
Aspiration.
The purpose of crosswise aspiration is to eliminate
residual sample or reagent from the wall perimeter. If the
buffer is water without any wetting agent (such as
Tween
®
20), crosswise aspiration might aid in removing
higher than expected residual.
Crosswise aspiration is used only with flat-bottom plates.
Lower residuals can often be achieved without crosswise
aspiration if the horizontal aspiration position is
programmed correctly.
Select
YES to designate a second aspiration.
Example of initial and then crosswise aspiration in a
microwell:
Horizontal
Aspirate
Position 10 Crosswise
Horizontal
Position -40
Crosswise On
Specify how the
Crosswise Aspiration shall
be performed.
All Select ALL to perform a crosswise
aspiration on all aspirations within the
wash program, or FINAL to perform the
crosswise aspiration on just the final
aspiration.
Crosswise Height
See the description for
Aspirate Height on the
preceding page.
All The only difference is that Crosswise
Height specifically refers to Crosswise
Aspiration.
Crosswise Horiz Pos
See the description for
Horizontal Aspr Pos on
the preceding page.
All The only difference is that Crosswise
Horizontal Position specifically refers to
Crosswise Aspiration. The recommended
range is +10 to +35.
Note: The shaded options above are not available for Aspirate-only programs.
Bio-Tek Instruments, Inc.