Bio-Tek ELx405, Руководство оператора

Страница: 86 / 250

56
| Chapter 3: Operation
Define Wash DISPENSE (DISP) Program Options
Option & Description Models Action/Comments
ELx405,
Magna,
Select,
Select CW,
HT/96-tube
The volume range is 50 to 3,000 µ l/well. Dispense Volume
The volume (in
microliters) of wash
solution to dispense per
microwell, per wash
cycle.
HT/192-tube The volume range is 25 to 3,000 µ l/well.
All The flow rate options range from 1 to 9,
where 1 = Slowest, and 9 = Fastest.
Dispense Flow Rate
The rate at which the
fluid is dispensed from
the tubes.
Select CW Two additional flow rates, 10 and 11, are
provided for gentle washing. Rates 1 to 9
range from lowest to highest flow. Rate
10 is lower than rate 1. Rate 11 is higher
than 10.
Note: Rates 10 and 11 are not
recommended for 384-well plates.
Performance of rates 10 and 11 may vary,
depending upon the plate and buffer
used.
Vacuum on Volume
The vacuum pump turns
on when the defined
volume is reached.
Select CW Appears only if flow rates 10 or 11 have
been selected for the Dispense Flow Rate
(see above). The volume range is 50 to
3000 µ l/well.
Bio-Tek recommends that you set the
vacuum on volume equal to your dispense
volume for small volumes. Refer also to
application notes on the Bio-Tek website
for more information (www.biotek.com).
All The options range from 12 to 180. 12 is
the closest to the carrier surface; 180 is
the furthest. The washer display shows
the corresponding measurement in
millimeters for each option.
Note: Due to
hardware variations, the 180 value may
be out of range for some washers; it is
recommended, therefore, that the highest
value be closer to 175 .
Dispense Height
(Overflow Position)
The height between the
bottom of the aspirate
tubes and the carrier
surface on which the
plate rests during the
dispense part of a wash
cycle. The dispense tubes
are shorter than the
aspirate tubes. See
Figures 18 to 20 .
Note: When dispensing volumes greater than the well
capacity, set a dispense height that positions the aspirate
tubes at the top of wells to draw off any overflow.
To visually determine the best offset parameters for a
microplate or assay, use the Adjust Utility (see page
92).
Bio-Tek Instruments, Inc.