Bio-Tek ELx405, Руководство оператора

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150
| Chapter 5: Performance Verification/Qualification Tests
Evacuation Efficiency Test (96)
This test is designed for Single/96 or Dual/96 manifolds. See page
157 if you’re looking for the Evacuation Efficiency Test for the
Dual/192 manifold.
See page 148 for a list of required materials.
1. Fill a supply bottle with two liters of deionized water. Run the Maintenance
program
DAY_RINSE two or three times to prime the fluid lines and
manifold.
2. Place a clean, dry 96-well microplate on the balance and zero the balance.
3. Pipette or dispense 150
µ
l of Solution #1 into each well of the microplate.
4. Place the plate on the washer and run the Aspirate program
residual_qc_test . This program evacuates all of the wells, leaving a
small amount of residual fluid.
5. When the program is finished, remove the plate and weigh it immediately,
because evaporation will affect the results. This is the
Total Residual
Weight
,
in grams.
6. Visually inspect the plate and note if any wells appear to have considerably
more liquid in them than others.
7. Using the
Evacuation Efficiency Test Worksheet
on page 164, perform
initial data reduction:
a. Divide the Total Residual Weight by
96
to find the
Mean Residual
Weight .
b. The Mean Residual Weight should be
<=
0.005 g
.
If the Mean Residual Weight is greater than 0.005 g, or if one or more wells appear
to have much more liquid than the others, the washer failed the test.
Troubleshoot as follows:
•
If the test fails once: Failure of this test is commonly caused by improper
aspiration tube placement within the wells, usually because a microplate
other than the recommended Corning Costar
®
96 was used.
Bio-Tek Instruments, Inc.