Bio-Tek ELx405, Руководство оператора

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Liquid Tests
|
151
If you prefer to use a plate other than the Corning Costar 96, modifying
the ASPIRATE HEIGHT or HORIZONTAL ASPIRATE POSITION
parameters in a copy of the residual_qc_test program should correct
this error ( residual_qc_test is locked for editing, but you can copy the
program and edit its parameters in the newly created copy). See
Chapter 3
,
Operation
, for instructions on copying and editing
programs and modifying the abovementioned parameters. After making
this change, retry the test using a clean, dry microplate.
If you are using the Corning Costar 96 microplate, run AUTOCLEAN
(Ultrasonic Advantage™ models only), and/or remove the manifold and
clean the aspiration tubes with the stylus (all models). See
Chapter 4
,
Preventive Maintenance
for complete instructions. When finished,
retry the test using a clean, dry microplate.
•
If the test fails a second time: Perform the
Supplemental Test
below.
Supplemental Test
Conduct this test if the Standard Evacuation Efficiency Test fails twice. This test will confirm
which aspirate tube(s) may be clogged, or if the plate’s alignment or position is the problem.
1. Repeat steps 2 through 7 of the previous test, using Solution #2 for
the dispense fluid. Be sure to recalculate the
Mean Residual Weight
.
2. Pipette 300
µ
l of Solution #1 into each well, on top of the residual
solution.
3. Shake the plate to get uniform distribution of the remaining dye in each
well.
If you are using one of Bio-Tek’s keypad-based readers, such as the
ELx800
 or ELx808  , ensure that the reader is not running in Rapid
mode . To check the setting, select
UTIL READ
and cycle through
the options until
READ IN RAPID MODE?
appears. Set it to NO .
4.
Read the plate in an optical reader (blank on air), using the
dual-wavelength method (630 nm - 450 nm), then print or export the
results.
5. Using the
Evacuation Efficiency Test Worksheet
on page 164,
perform data reduction:
a. Calculate the sum of the OD values for all 96 wells, then divide by
96 to determine the Mean OD for the plate.
b. Divide the Mean OD by the Mean Residual Weight (from step 1),
to find the Residual Factor
.
ELx405 Operator's Guide