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5.15. Conditions of use ............................................................................................................. 22
5.16. Description of the operator's workplace .......................................................................... 22
5.17. Personal protective equipment ........................................................................................ 22
5.18. Restrictions on the use of the Autosampler III ................................................................. 22
6. Standard operating procedure - Autosampler III ......................................................................... 23
6.1. Prepare the compressor for use: ........................................................................................ 23
6.1.1. Starting up the compressor: ...................................................................................... 23
6.1.2. Shutting down of the compressor: ............................................................................ 25
6.2. Starting up the Autosampler III ........................................................................................... 25
6.3. Initializing the Autosampler III ............................................................................................. 27
6.3.1. Checklist prior to starting Autosampler III initialization ............................................. 27
6.4. Starting Autosampler III initialization .................................................................................. 32
6.5. Priming the pumps ............................................................................................................. 33
6.5.1. Checklist prior to start priming the pumps ................................................................ 33
6.5.2. Start priming method ................................................................................................. 34
6.6. Loading plates .................................................................................................................... 34
6.7. Pre-Run .............................................................................................................................. 36
6.8. Lock/Unlock doors ............................................................................................................. 36
6.9. Starting a run ...................................................................................................................... 37
6.10. Main run ............................................................................................................................ 38
6.11. Dummies .......................................................................................................................... 39
6.12. Manual PO ........................................................................................................................ 40
6.13. Exchange of liquids procedure ......................................................................................... 42
6.13.1. Pumps ..................................................................................................................... 42
6.13.2. Chemical containers ................................................................................................ 43
6.14. End of day procedure ....................................................................................................... 48
7. Daily validation procedure ........................................................................................................... 49
7.1. Autosampler III checks prior to starting daily validation..................................................... 49
7.2. Starting the daily validation ................................................................................................ 49
8. Replacing needle(s) ..................................................................................................................... 50
9. Cleaning the Autosampler III ....................................................................................................... 52
9.1. Outside of the Autosampler III ............................................................................................ 52
9.1.1. See-through parts such as doors, windows and covers ........................................... 52
9.1.2. Trespa panels (white) ................................................................................................. 52
9.1.3. Frame parts ............................................................................................................... 52
9.1.4. Pipetting cabin (powder coated white) ...................................................................... 52
9.1.5. Deck/stainless steel ................................................................................................... 53
9.1.6. Barcode camera ........................................................................................................ 53
9.1.7. HMI ............................................................................................................................ 53
