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CORN I NG ASCENT FBR PD SYSTEM
6.4.4 Cell Removal
This phase transports the released cells and solution from the flush bottle through the bioreactor and into the collection bottle by
pressurizing the system via the pressure control valve inside the instrument. Air pressure can be set at approximately 10 to 15 psi.
The specified air pressure is first applied to the flush bottle during Pressurization Delay allowing time to build up pressure. Pressurized
air is flushed out of the flush bottle through the bioreactor into the collection bottle during cell removal. When pressure is applied to the
flush bottle for the Removal Duration, harvest solution is flowed at around 2L/min. through the bioreactor into the collection bottle. The
goal of fast flow through the bioreactor is to retrieve most of the cells into the collection bottle. Figure 6-6 highlights the cell removal
path. Table 6-17 shows the setpoints controlled.
NOTE:
Cell Release and Cell Removal Phases can be repeated to achieve complete cell or product harvest.
Table 6-17.
Cell removal setpoints.
Setpoints
Harvest Cell Removal Air Pressure
Harvest Cell Removal Pressurization Delay
Harvest Cell Removal Duration
Figure 6-5.
Cell release path highlighted.
Figure 6-6.
Cell removal path highlighted.
