125
Instruction Manual
User Action
System Auto Sequence
User confirms the MCV Volume and that media
addition is complete or manually jogs pump,
then confirms prompt to complete media
addition.
User updates MCV Volume if needed.
De-energizes red pinch valve.
Restarts equipment module loops as follows:
MCV DO
Starts Stabilization Delay Duration timer and MCV DO and gas control
equipment module with output at value recorded. When timer has
elapsed, resumes normal automated control of equipment module
setpoints.
FBR DO
When Stabilization Delay Duration Timer from the MCV DO loop has
elapsed, resumes automated control of equipment module setpoints.
Temperature
Starts MCV Temperature and FBR Temperature control equipment
modules with both of their outputs held at the recorded controller
values until the Stabilization Delay Duration has elapsed. When
timer has elapsed, resumes normal automated control of equipment
module setpoints.
pH
Reads output value recorded.
If recorded output is >50: Starts equipment module pH control with
its output held at 50% until Stabilization timer has elapsed. When
timer has elapsed resumes normal automated control of equipment
module setpoint.
If recorded output is ≤50: Starts equipment module pH control with
its output held at the value recorded until Stabilization timer has
elapsed. When timer has elapsed resumes normal automated control
of equipment module setpoint.
When Stabilization Delay Duration timer has elapsed, notifies the
user that Media Exchange was completed.
User confirms that Media Exchange is
complete.
N/A
User Action
System Auto Sequence
Case C. MCV Volume is ≥2.75L and the Media
Removal Volume is ≥FBRX Loop Volume
Starts media pump counterclockwise at the Removal Flow Rate set-
point until the FBR Loop Volume is reached at the totalizer. Do not
clear phase totalizer.
Sets recirculation pump to Operator mode.
Prompts user to manually jog recirculation pump in reverse to empty
the bioreactor to the desired level, and to confirm when complete.
User manually jogs the pump in reverse
to empty the FBR to the desired level, and
acknowledges prompt when complete.
Starts the media pump counterclockwise at the Removal Flow Rate
setpoint until the Media Pump Totalizer reaches Media Removal
Volume setpoint.
Subtracts the Removal Volume from the Current MCV Volume and
Current Total System Volume.
Sets media pump to Operator mode.
Prompts user to jog media pump to add/remove desired volume from MCV.
Media Exchange Phase
