12
CORN I NG ASCENT FBR PD SYSTEM
5.2.1 Agitator Motor
The motor driven Rushton impeller uniformly mixes the media in the MCV. The magnetically coupled agitator motor is manually mounted
on the MCV headplate (Figure 5-14) to drive the agitator shaft (Figure 5-3). Agitation speed can be controlled manually or automatically to
maintain proper level of media oxygenation in the MCV.
The recommended agitation operational range is 0 to 400 rpm, and the speed can be set up to 500 rpm via the HMI.
NOTE:
It is not recommended that the agitator motor cable be disconnected from either end as motor malfunction may occur. Motor
should be kept in the motor holder when not being used on an MCV (see Figure 5-14).
Figure 5-14.
Agitator motor
connection is plugged into
the interface panel. To start or
stop the agitator, the motor is
manually mounted or removed
from the MCV headplate.
Label
Interface Panel
Description
Reference
1
Agitator motor
Agitator motor cable
Section 5.2.1
2
Filter heater
Exhaust filter heater connection
Section 5.2.2
3
MCV pressure
In-line sensor that monitors pressure on the MCV gas vent
Section 5.2.3
4
MCV sparge
Sparging gas line in the MCV
Section 5.2.4
5
MCV temperature
In-line sensor monitors the media temperature exiting the
MCV via the recirculation loop
Section 5.2.5
6
FBR DO (DO2)
Monitors dissolved oxygen levels exiting the bioreactor
Section 5.2.6
7
MCV pH
Monitors pH levels exiting the MCV
Section 5.2.7
8
MCV DO (DO1)
Monitors dissolved oxygen levels exiting the MCV
Section 5.2.6
Table 5-7
. Interface panel ports.
5.2.2 Exhaust Filter Heater
An exhaust line (Figure 5-15) prevents pressure build-up in the MCV. The exhaust line has a filter that requires heating to prevent
condensate build-up. Heating is performed via a reusable silicone heater blanket that keeps the filter dry. The heater blanket is manually
secured via buttons around the filter element before a process run so that the filter is immediately heated when gas flow begins. The
heater temperature is controlled to a fixed power output. A built-in thermostat prevents the temperature from rising above 65°C. A wire
rack keeps the exhaust line with the secured heater blanket supported.
NOTE:
The vent filter heater is only turned off at system shutdown. It remains on during harvest and in situ lysis.
Figure 5-15.
Heater blanket
wrapped around the exhaust
filter on the gas line.
