6
CORN I NG ASCENT FBR PD SYSTEM
Table 5-2.
Tubing and ports on the MCV headplate.
Label
Description
IN
OUT
T1
Not used.
T2
Adds and removes media from the MCV.
T3
Channels MCV media through the sensor tray and sterile connections to the
bioreactor. The tubing has a sensor that monitors the MCV temperature.
T4
Returns media from the bioreactor. There are two features on the line:
• Media sampling port.
• Valve is manually opened when draining the bioreactor (e.g., at substrate
sampling and media exchange). Valve default position is closed.
T5
Channels transfection agent and inoculum aseptically to the MCV.
T6
Tubing line with red and blue clips to the feed bottle. Feed is added to the
MCV via the feed pump.
T7
Tubing line with yellow and blue clips to the base bottle. Base is added to the
MCV via the base pump.
p1
Auxiliary Luer-Lock port.
p2
Media sampling Luer-Lock port.
Figure 5-2.
MCV headplate connections
for media maintenance, transfection,
inoculation, and media sampling
(see Table 5-2).
Figure 5-2 shows the MCV headplate connections, tubing lines, and ports.
