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CORN I NG ASCENT FBR PD SYSTEM
Appendix 2: Phase Permissive
Table A2-1 shows the system’s phase permissive which places select devices in Program mode when phases start. If the phase was in
Operator mode at phase start, select devices are placed in Program mode.
Table A2-1.
Phase permissives.
Phase
Automated Phase Permissive/Devices Put into Program Mode
Initialize
All devices and equipment modules.
Media Fill/Prime
Orange and red pinch valves, gas shutoff valve, recirculation pump, and media pump.
Media Conditioning
Gas shutoff valve, recirculation pump, air mass flow controller, O
2
mass flow controller, CO
2
mass flow
controller, agitator, MCV temperature equipment module, and FBR Temperature equipment module.
Media Maintenance
Gas shutoff valve, all mass flow controllers, recirculation pump, base pump, agitator, and all equipment
modules.
Inoculation
Gas shutoff valve, recirculation pump, base pump, media pump, air mass flow controller, agitator, and all
equipment modules.
Media Exchange
Orange and red pinch valves, gas shutoff valve, recirculation pump, media pump, all mass flow controllers,
and all equipment modules.
Transfection
Gas shutoff valve, recirculation pump, feed pump, all mass flow controllers, agitator, and all equipment
modules.
Harvest Prep
All devices and equipment modules.
Harvest Wash
Yellow, blue, and green pinch valves, harvest pressure valve, gas shutoff valve, and recirculation pump.
Cell Release
Yellow, blue, and green pinch valves, harvest pressure valve, gas shutoff valve, pressure control valve, and
recirculation pump.
Cell Removal
Yellow, blue, and green pinch valves, harvest pressure valve, gas shutoff valve, and pressure control valve.
Final Rinse
Yellow, blue, and green pinch valves, harvest pressure valve, gas shutoff valve, pressure control valve, and
recirculation pump
Shutdown
All devices and equipment modules.
Substrate Sampling
All pumps, all mass flow controllers, agitator, and all equipment modules.
Media Addition
Orange and red pinch valves, and media pump.
Bolus Addition
Feed pump.
Media Removal
Orange and red pinch valves, and media pump.
Perfusion
Orange and red pinch valves, and media pump.
Dilution
Orange and red pinch valves, and media pump.
Equipment module refers to equipment controlled by the control loop together to control a process value such as temperature or pH.
Table A2-2 shows the five equipment modules, and lists the devices in program mode when each equipment module is in program mode.
Table A2-2.
Devices placed in program mode when equipment module is in program mode.
Equipment Module in Program Mode
Devices in Program Mode
MCV DO
Air mass flow controller, O
2
mass flow controller, agitator.
FBR DO
Recirculation pump.
pH
CO
2
mass flow controller, base pump.
MCV Temperature
MCV heater.
FBR Temperature
FBR heater.
