9
Instruction Manual
The bioreactor has a sanitary twist clamp that allows easy access to the segmented sampling mesh located below (Figure 5-8). The 3
sampling mesh disks (Figure 5-9) are divided into six segments, which are designed for easy removal. Segments may be removed to
monitor cell growth in the FBR.
Figure 5-6.
FBR and Lynx® connectors.
Figure 5-7.
FBR and AseptiQuik® G connectors.
Figure 5-9.
A segmented sampling mesh. There
are 3 segmented sampling meshes, each with 6
segments, totaling 18 sampling segments. One
or more segments may be removed each day for
sampling.
5.1.6 Harvest Kit
The Corning® Ascent™ FBR system has a consumable harvest kit in a portable tray for easy positioning enabling users to recover cells from
the bioreactor (Figure 5-10).
Prior to harvest, the bioreactor is fluidically isolated from the MCV by clamping the recirculation inlet, and the outlet tubing. During the
Harvest Phase, the recirculation pump, in conjunction with the automated pinch valves, perfuse harvesting solutions from the harvest kit
through the bioreactor. See Table 5-4 for harvest kit descriptions.
Figure 5-10.
Illustration showing the harvest kit.
The kit is only set up at harvest.
Figure 5-8.
The growth mesh
stack and sampling mesh
layers are removable from
the bioreactor vessel.
