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CORN I NG ASCENT FBR PD SYSTEM
User Action
System Auto Sequence
User confirms the MCV volume and that media
addition is complete or manually jogs pump,
then confirms prompt to complete media
addition.
User updates MCV Volume if needed.
De-energizes the red pinch valve.
Restarts equipment module loops as follows:
MCV DO
Starts Stabilization Delay Duration timer and MCV DO and Gas
Control equipment modules with output at the value recorded. When
timer has elapsed, resumes normal automated control of equipment
module setpoints.
FBR DO
When stabilization delay duration timer from the MCV DO loop has
elapsed, resumes automated control of equipment module setpoints.
Temperature
Starts the MCV Temperature and FBR Temperature control equipment
modules with both of their outputs held at the recorded controller
values until the Stabilization Delay Duration has elapsed. When
timer has elapsed, resumes normal automated control of equipment
module setpoints.
pH
Reads output value recorded.
If recorded output is >50: Starts equipment module pH control with
its output held at 50% until Stabilization timer has elapsed. When
timer has elapsed resumes normal automated control of equipment
module setpoint.
If recorded output is ≤50: Starts equipment module pH control with
its output held at the value recorded until Stabilization timer has
elapsed. When timer has elapsed resumes normal automated control
of equipment module setpoint.
When Stabilization Delay Duration timer has elapsed, notifies the
user that Media Exchange was completed.
User confirms that Media Exchange is
complete.
N/A
User Action
System Auto Sequence
Case B. MCV Volume is ≥2.75L and the Media
Removal Volume is <FBR Loop Volume
Starts media pump counterclockwise at the Removal Flow Rate
setpoint until the Media Pump Totalizer reaches Media Removal
Volume setpoint.
Subtracts the Removal Volume from the Current MCV Volume and
Current Total System Volume.
Sets media pump to Operator mode.
Prompts user to jog media pump to add/remove desired volume
from MCV.
User acknowledges prompt to jog media pump. Resets phase totalizer for media and recirculation pumps.
Energizes Red pinch valve to the media reservoir.
Starts media pump clockwise at the Media Pump Addition Flow Rate
setpoint.
When Current MCV Volume reaches (Minimum MCV Volume plus
Bioreactor Loop Volume), starts recirculation pump clockwise at the
Recirculation Pump Addition Flow Rate until the Recirculation Pump
Totalizer reaches the Bioreactor Loop Volume. When Bioreactor Loop
Volume is reached, ramps recirculation pump to the controller value
recorded over 120 seconds.
Continues to run the media pump at the Media Addition Flow Rate
setpoint until the Media Pump Totalizer reaches the Media Addition
Volume setpoint.
Sets media pump to Operator mode.
Adds the volume from Media Pump Totalizer to the Total System
Volume. Subtracts the Bioreactor Loop Volume from the Total System
Volume to calculate Current MCV Volume.
Prompts user to confirm displayed Current MCV Volume and that
media addition is complete or jog media pump until complete.
Media Exchange Phase
