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Instruction Manual
15. Connect the sensor tray in-line sensors.
• Tilt the sensor sideways prior to install to prevent air bubbles.
• Loosen each colored cap on the sensor tray.
• Insert the in-line sensor probe from the interface panel to the color matched sensors on the
sensor tray.
• Twist and turn the colored cap to tighten. Make sure the probe sits flush with the end of
each sensor.
MCV DO sensor: Blue clip and cap
FBR DO sensor: Yellow clip and blue cap
MCV pH sensor: Red clip and cap
NOTE:
Care must be taken to first ensure that inserting the fiber optic guide does not result
in the black coating on the guide flaring. If the coating flares or pushes back from the end of
the fiber, insertion will become difficult. Second, care must be taken to ensure the fiber optic
guide is seated all the way at the bottom of the sensor housing. Failure to seat the fiber optic
guide will result in unreliable measurements.
• Close the clamp at the AseptiQuik® G connection above the sensor tray.
• Make sure the clamps at the sensor tray are not causing kinks in the media lines.
• It may be necessary to turn/twist the sensor tray if the media lines are crossed.
• The sensor tray and interface panel connections are now set up.
• Proceed to connect the MCV and FBR.
16. MCV to FBR sterile connections.
For instruments using Lynx® Connectors, go to Section 8.2.1.1.
For instruments using AseptiQuik® G connectors, go to Section 8.2.1.2.
• Once the sterile connections between the MCV and FBR area connected, check that the FBR is
correctly seated.
• Before placing the tray over the bioreactor nest, check that the bioreactor pin (pointed rigid
feature) is visible.
17. Check for tubing kinks.
The tubing lines under the tray must not be twisted or kinked.
TIP 1:
Rotate the bioreactor in the nest to untwist the bioreactor inlet tubing.
TIP 2:
Untwist the in-line temperature sensor tubing if kinked around the barb connector.
